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Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primates.

dc.contributor.author Chen, Benny J
dc.contributor.author Deoliveira, Divino
dc.contributor.author Spasojevic, Ivan
dc.contributor.author Sempowski, Gregory D
dc.contributor.author Jiang, Chen
dc.contributor.author Owzar, Kouros
dc.contributor.author Wang, Xiaojuan
dc.contributor.author Gesty-Palmer, Diane
dc.contributor.author Cline, J Mark
dc.contributor.author Bourland, J Daniel
dc.contributor.author Dugan, Greg
dc.contributor.author Meadows, Sarah K
dc.contributor.author Daher, Pamela
dc.contributor.author Muramoto, Garrett
dc.contributor.author Chute, John P
dc.contributor.author Chao, Nelson J
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:31Z
dc.date.issued 2010-06-16
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20585403
dc.identifier.uri https://hdl.handle.net/10161/4547
dc.description.abstract Medications that can mitigate against radiation injury are limited. In this study, we investigated the ability of recombinant human growth hormone (rhGH) to mitigate against radiation injury in mice and nonhuman primates. BALB/c mice were irradiated with 7.5 Gy and treated post-irradiation with rhGH intravenously at a once daily dose of 20 microg/dose for 35 days. rhGH protected 17 out of 28 mice (60.7%) from lethal irradiation while only 3 out of 28 mice (10.7%) survived in the saline control group. A shorter course of 5 days of rhGH post-irradiation produced similar results. Compared with the saline control group, treatment with rhGH on irradiated BALB/c mice significantly accelerated overall hematopoietic recovery. Specifically, the recovery of total white cells, CD4 and CD8 T cell subsets, B cells, NK cells and especially platelets post radiation exposure were significantly accelerated in the rhGH-treated mice. Moreover, treatment with rhGH increased the frequency of hematopoietic stem/progenitor cells as measured by flow cytometry and colony forming unit assays in bone marrow harvested at day 14 after irradiation, suggesting the effects of rhGH are at the hematopoietic stem/progenitor level. rhGH mediated the hematopoietic effects primarily through their niches. Similar data with rhGH were also observed following 2 Gy sublethal irradiation of nonhuman primates. Our data demonstrate that rhGH promotes hematopoietic engraftment and immune recovery post the exposure of ionizing radiation and mitigates against the mortality from lethal irradiation even when administered after exposure.
dc.language eng
dc.language.iso en_US
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0011056
dc.subject Animals
dc.subject Apoptosis
dc.subject Growth Hormone
dc.subject Mice
dc.subject Mice, Inbred BALB C
dc.subject Mice, Inbred C57BL
dc.subject Primates
dc.subject Radiation Injuries, Experimental
dc.subject Recombinant Proteins
dc.subject T-Lymphocyte Subsets
dc.title Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primates.
dc.title.alternative
dc.type Journal article
duke.contributor.id Chen, Benny J|0183118
duke.contributor.id Spasojevic, Ivan|0104047
duke.contributor.id Sempowski, Gregory D|0198755
duke.contributor.id Owzar, Kouros|0298315
duke.contributor.id Gesty-Palmer, Diane|0122004
duke.contributor.id Chute, John P|0314438
duke.contributor.id Chao, Nelson J|0137379
dc.description.version Version of Record
duke.date.pubdate 2010-6-16
duke.description.issue 6
duke.description.volume 5
dc.relation.journal Plos One
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20585403
pubs.begin-page e11056
pubs.issue 6
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biostatistics & Bioinformatics
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Human Vaccine Institute
pubs.organisational-group Global Health Institute
pubs.organisational-group Immunology
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cellular Therapy
pubs.organisational-group Medicine, Duke Human Vaccine Institute
pubs.organisational-group Medicine, Endocrinology, Metabolism, and Nutrition
pubs.organisational-group Medicine, Medical Oncology
pubs.organisational-group Pathology
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 1932-6203
duke.contributor.orcid Sempowski, Gregory D|0000-0003-0391-6594


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