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Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profiles.

dc.contributor.author Meadows, Sarah K
dc.contributor.author Dressman, Holly K
dc.contributor.author Daher, Pamela
dc.contributor.author Himburg, Heather
dc.contributor.author Russell, J Lauren
dc.contributor.author Doan, Phuong
dc.contributor.author Chao, Nelson J
dc.contributor.author Lucas, Joseph
dc.contributor.author Nevins, Joseph R
dc.contributor.author Chute, John P
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:32Z
dc.date.issued 2010-07-12
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20634956
dc.identifier.uri https://hdl.handle.net/10161/4550
dc.description.abstract In the event of a terrorist-mediated attack in the United States using radiological or improvised nuclear weapons, it is expected that hundreds of thousands of people could be exposed to life-threatening levels of ionizing radiation. We have recently shown that genome-wide expression analysis of the peripheral blood (PB) can generate gene expression profiles that can predict radiation exposure and distinguish the dose level of exposure following total body irradiation (TBI). However, in the event a radiation-mass casualty scenario, many victims will have heterogeneous exposure due to partial shielding and it is unknown whether PB gene expression profiles would be useful in predicting the status of partially irradiated individuals. Here, we identified gene expression profiles in the PB that were characteristic of anterior hemibody-, posterior hemibody- and single limb-irradiation at 0.5 Gy, 2 Gy and 10 Gy in C57Bl6 mice. These PB signatures predicted the radiation status of partially irradiated mice with a high level of accuracy (range 79-100%) compared to non-irradiated mice. Interestingly, PB signatures of partial body irradiation were poorly predictive of radiation status by site of injury (range 16-43%), suggesting that the PB molecular response to partial body irradiation was anatomic site specific. Importantly, PB gene signatures generated from TBI-treated mice failed completely to predict the radiation status of partially irradiated animals or non-irradiated controls. These data demonstrate that partial body irradiation, even to a single limb, generates a characteristic PB signature of radiation injury and thus may necessitate the use of multiple signatures, both partial body and total body, to accurately assess the status of an individual exposed to radiation.
dc.language eng
dc.language.iso en_US
dc.publisher Public Library of Science (PLoS)
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0011535
dc.subject Animals
dc.subject Female
dc.subject Gene Expression
dc.subject Gene Expression Profiling
dc.subject Leukocytes, Mononuclear
dc.subject Mice
dc.subject Mice, Inbred C57BL
dc.subject Oligonucleotide Array Sequence Analysis
dc.subject Radiation Injuries, Experimental
dc.subject Radiation, Ionizing
dc.subject Whole-Body Irradiation
dc.title Diagnosis of partial body radiation exposure in mice using peripheral blood gene expression profiles.
dc.title.alternative
dc.type Journal article
duke.contributor.id Dressman, Holly K|0239213
duke.contributor.id Himburg, Heather|0280633
duke.contributor.id Doan, Phuong|0110004
duke.contributor.id Chao, Nelson J|0137379
duke.contributor.id Lucas, Joseph|0308238
duke.contributor.id Nevins, Joseph R|0084291
duke.contributor.id Chute, John P|0314438
dc.description.version Version of Record
duke.date.pubdate 2010-7-12
duke.description.issue 7
duke.description.volume 5
dc.relation.journal Plos One
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20634956
pubs.begin-page e11535
pubs.issue 7
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biostatistics & Bioinformatics
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Faculty
pubs.organisational-group Global Health Institute
pubs.organisational-group Immunology
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cellular Therapy
pubs.organisational-group Medicine, Hematological Malignancies
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Pathology
pubs.organisational-group School of Medicine
pubs.organisational-group Social Science Research Institute
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 1932-6203
duke.contributor.orcid Doan, Phuong|0000-0003-1361-2068


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