The effects of sleep hypoxia on coagulant factors and hepatic inflammation in emphysematous rats.
Abstract
OBJECTIVES: To develop a sleep hypoxia (SH) in emphysema (SHE) rat model and to explore
whether SHE results in more severe hepatic inflammation than emphysema alone and whether
the inflammation changes levels of coagulant/anticoagulant factors synthesized in
the liver. METHODS: Seventy-five rats were put into 5 groups: SH control (SHCtrl),
treated with sham smoke exposure (16 weeks) and SH exposure (12.5% O(2), 3 h/d, latter
8 weeks); emphysema control (ECtrl), smoke exposure and sham SH exposure (21% O(2));
short SHE (SHEShort), smoke exposure and short SH exposure (1.5 h/d); mild SHE (SHEMild),
smoke exposure and mild SH exposure (15% O(2)); standard SHE (SHEStand), smoke exposure
and SH exposure. Therefore, ECtrl, SHEShort, SHEMild and SHEStand group were among
emphysematous groups. Arterial blood gas (ABG) data was obtained during preliminary
tests. After exposure, hepatic inflammation (interleukin -6 [IL-6] mRNA and protein,
tumor necrosis factor α [TNFα] mRNA and protein) and liver coagulant/anticoagulant
factors (antithrombin [AT], fibrinogen [FIB] and Factor VIII [F VIII]) were evaluated.
SPSS 11.5 software was used for statistical analysis. RESULTS: Characteristics of
emphysema were obvious in emphysematous groups and ABGs reached SH criteria on hypoxia
exposure. Hepatic inflammation parameters and coagulant factors are the lowest in
SHCtrl and the highest in SHEStand while AT is the highest in SHCtrl and the lowest
in SHEStand. Inflammatory cytokines of liver correlate well with coagulant factors
positively and with AT negatively. CONCLUSIONS: When SH is combined with emphysema,
hepatic inflammation and coagulability enhance each other synergistically and produce
a more significant liver-derivative inflammatory and prothrombotic status.
Type
Journal articleSubject
AnimalsBase Sequence
Coagulants
DNA Primers
Electroencephalography
Emphysema
Hepatitis, Animal
Hypoxia
Interleukin-6
Male
RNA, Messenger
Rats
Rats, Wistar
Sleep
Tumor Necrosis Factor-alpha
Permalink
https://hdl.handle.net/10161/4574Published Version (Please cite this version)
10.1371/journal.pone.0013201Publication Info
Feng, Jing; Wang, Qing-shan; Chiang, Ambrose; & Chen, Bao-yuan (2010). The effects of sleep hypoxia on coagulant factors and hepatic inflammation in emphysematous
rats. PLoS One, 5(10). pp. e13201. 10.1371/journal.pone.0013201. Retrieved from https://hdl.handle.net/10161/4574.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
Collections
More Info
Show full item recordScholars@Duke
Ambrose An-Po Chiang
Associate Professor of Medicine
Nocturnal tachypnea in OSA patients; CPAP compliance in patients with OSA; Relationship
between non-alcoholic fatty liver disease and OSA; Post-operative care in patients
with sleep apnea; Cheyne-Stokes Respiration and atrial fibrillation

Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy
Rights for Collection: Scholarly Articles
Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info