Profiles of human serum antibody responses elicited by three leading HIV vaccines focusing on the induction of Env-specific antibodies.
Abstract
In the current report, we compared the specificities of antibody responses in sera
from volunteers enrolled in three US NIH-supported HIV vaccine trials using different
immunization regimens. HIV-1 Env-specific binding antibody, neutralizing antibody,
antibody-dependent cell-mediated cytotoxicity (ADCC), and profiles of antibody specificity
were analyzed for human immune sera collected from vaccinees enrolled in the NIH HIV
Vaccine Trial Network (HVTN) Study #041 (recombinant protein alone), HVTN Study #203
(poxviral vector prime-protein boost), and the DP6-001 study (DNA prime-protein boost).
Vaccinees from HVTN Study #041 had the highest neutralizing antibody activities against
the sensitive virus along with the highest binding antibody responses, particularly
those directed toward the V3 loop. DP6-001 sera showed a higher frequency of positive
neutralizing antibody activities against more resistant viral isolate with a significantly
higher CD4 binding site (CD4bs) antibody response compared to both HVTN studies #041
and #203. No differences were found in CD4-induced (CD4i) antibody responses, ADCC
activity, or complement activation by Env-specific antibody among these sera. Given
recent renewed interest in realizing the importance of antibody responses for next
generation HIV vaccine development, different antibody profiles shown in the current
report, based on the analysis of a wide range of antibody parameters, provide critical
biomarker information for the selection of HIV vaccines for more advanced human studies
and, in particular, those that can elicit antibodies targeting conformational-sensitive
and functionally conserved epitopes.
Type
Journal articleSubject
AIDS VaccinesAntibodies, Neutralizing
Antibody Specificity
Antibody-Dependent Cell Cytotoxicity
Enzyme-Linked Immunosorbent Assay
HIV Antibodies
HIV Envelope Protein gp120
HIV Infections
HIV-1
Humans
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https://hdl.handle.net/10161/4583Published Version (Please cite this version)
10.1371/journal.pone.0013916Publication Info
Vaine, Michael; Wang, Shixia; Liu, Qin; Arthos, James; Montefiori, David; Goepfert,
Paul; ... Lu, Shan (2010). Profiles of human serum antibody responses elicited by three leading HIV vaccines
focusing on the induction of Env-specific antibodies. PLoS One, 5(11). pp. e13916. 10.1371/journal.pone.0013916. Retrieved from https://hdl.handle.net/10161/4583.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
David Charles Montefiori
Professor in Surgery
Dr. Montefiori is Professor and Director of the Laboratory for HIV and COVID-19 Vaccine
Research & Development in the Department of Surgery, Division of Surgical Sciences
at Duke University Medical Center. His major research interests are viral immunology
and HIV and COVID-19 vaccine development, with a special emphasis on neutralizing
antibodies. Multiple aspects of HIV-1 neutralizing antibodies are studied in his laboratory,
including mechanisms of neutralization and escape,

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