Show simple item record Ni, T Tu, K Wang, Z Song, S Wu, H Xie, B Scott, KC Grewal, SI Gao, Y Zhu, J
dc.coverage.spatial United States 2011-06-21T17:32:20Z 2010-12-20
dc.identifier.citation PLoS One, 2010, 5 (12), pp. e15271 - ?
dc.description.abstract A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe.
dc.format.extent e15271 - ?
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0015271
dc.subject DNA Polymerase II
dc.subject Gene Expression Profiling
dc.subject Gene Expression Regulation, Fungal
dc.subject Gene Library
dc.subject Genes, Dominant
dc.subject Genes, Fungal
dc.subject Genome, Fungal
dc.subject Histones
dc.subject Meiosis
dc.subject Models, Genetic
dc.subject Oligonucleotides, Antisense
dc.subject RNA
dc.subject Reverse Transcriptase Polymerase Chain Reaction
dc.subject Saccharomyces cerevisiae Proteins
dc.subject Schizosaccharomyces
dc.subject Sequence Analysis, DNA
dc.title The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-12-20 en_US
duke.description.endpage e15271 en_US
duke.description.issue 12 en_US
duke.description.startpage e15271 en_US
duke.description.volume 5 en_US
dc.relation.journal Plos One en_US
pubs.issue 12
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Molecular Genetics and Microbiology
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 1932-6203

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