Show simple item record Petersen, TH Hitchcock, T Muto, A Calle, EA Zhao, L Gong, Z Gui, L Dardik, A Bowles, DE Counter, CM Niklason, LE
dc.coverage.spatial United States 2011-06-21T17:32:24Z 2010
dc.identifier ct2075petersen
dc.identifier.citation Cell Transplant, 2010, 19 (1), pp. 79 - 87
dc.description.abstract While advances in regenerative medicine and vascular tissue engineering have been substantial in recent years, important stumbling blocks remain. In particular, the limited life span of differentiated cells that are harvested from elderly human donors is an important limitation in many areas of regenerative medicine. Recently, a mutant of the human telomerase reverse transcriptase enzyme (TERT) was described, which is highly processive and elongates telomeres more rapidly than conventional telomerase. This mutant, called pot1-TERT, is a chimeric fusion between the DNA binding protein pot1 and TERT. Because pot1-TERT is highly processive, it is possible that transient delivery of this transgene to cells that are utilized in regenerative medicine applications may elongate telomeres and extend cellular life span while avoiding risks that are associated with retroviral or lentiviral vectors. In the present study, adenoviral delivery of pot1-TERT resulted in transient reconstitution of telomerase activity in human smooth muscle cells, as demonstrated by telomeric repeat amplification protocol (TRAP). In addition, human engineered vessels that were cultured using pot1-TERT-expressing cells had greater collagen content and somewhat better performance in vivo than control grafts. Hence, transient delivery of pot1-TERT to elderly human cells may be useful for increasing cellular life span and improving the functional characteristics of resultant tissue-engineered constructs.
dc.format.extent 79 - 87
dc.language eng
dc.language.iso en_US en_US
dc.relation.ispartof Cell Transplant
dc.relation.isversionof 10.3727/096368909X478650
dc.subject Adenoviridae
dc.subject Adult
dc.subject Animals
dc.subject Bioreactors
dc.subject Blood Vessels
dc.subject Cell Aging
dc.subject Cell Culture Techniques
dc.subject Cells, Cultured
dc.subject Collagen
dc.subject Genetic Vectors
dc.subject Graft Survival
dc.subject Humans
dc.subject Male
dc.subject Muscle, Smooth, Vascular
dc.subject Rats
dc.subject Rats, Nude
dc.subject Recombinant Fusion Proteins
dc.subject Telomerase
dc.subject Telomere-Binding Proteins
dc.subject Tissue Engineering
dc.subject Transfection
dc.title Utility of telomerase-pot1 fusion protein in vascular tissue engineering.
dc.title.alternative en_US
dc.type Journal Article
dc.description.version Version of Record en_US 2010-00-00 en_US
duke.description.endpage 87 en_US
duke.description.issue 1 en_US
duke.description.startpage 79 en_US
duke.description.volume 19 en_US
dc.relation.journal Cell transplantation en_US
pubs.issue 1
pubs.organisational-group /Duke
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments
pubs.organisational-group /Duke/School of Medicine/Basic Science Departments/Pharmacology & Cancer Biology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Radiation Oncology
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Surgery
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Surgery/Surgery, Surgical Sciences
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers
pubs.organisational-group /Duke/School of Medicine/Institutes and Centers/Duke Cancer Institute
pubs.publication-status Published
pubs.volume 19
dc.identifier.eissn 1555-3892

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