Skip to main content
Duke University Libraries
DukeSpace Scholarship by Duke Authors
  • Login
  • Ask
  • Menu
  • Login
  • Ask a Librarian
  • Search & Find
  • Using the Library
  • Research Support
  • Course Support
  • Libraries
  • About
View Item 
  •   DukeSpace
  • Theses and Dissertations
  • Masters Theses
  • View Item
  •   DukeSpace
  • Theses and Dissertations
  • Masters Theses
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Modification of pH and pressure in a microfluidic cell culture device.

Thumbnail
View / Download
2.5 Mb
Date
2012
Author
Gatti, John WIlliam
Advisor
Yuan, Fan
Repository Usage Stats
323
views
259
downloads
Abstract

A three-dimensional tissue culture device previously described by Elliott et al. 1 is tested for its capacity to mimic cancer interstitial fluid pressure and interstitial pH. The device described by Elliott et al. is a three-channel system; a central channel that contains cells, and two side channels that act as model capillaries. The potential for variation of the interstitial fluid pressure was determined by measuring the resistances with respect to the various channels. It was determined that the unmodified device is incapable of mimicking physiological tumor pressures. The ability to vary pH was performed by using a pH sensitive florescent and by infusing a pH 5.9 solution into the side channels, before checking if the device could maintain this pressure for a long time period during constant perfusion of media into the device. It was observed that the florescence of the pH sensitive molecule decreased upon infusion of an acidic solution, as it should upon exposure to acid. However, no conclusions can be drawn from these tests, as the florescent molecule was no longer viable after the 12 hour perfusion of media. Further experiments are needed before any conclusions can be reached about the device's potential.

Type
Master's thesis
Department
Biomedical Engineering
Subject
Biomedical engineering
Cancer
Cell Culture
pH
Pressure
Permalink
https://hdl.handle.net/10161/5541
Citation
Gatti, John WIlliam (2012). Modification of pH and pressure in a microfluidic cell culture device. Master's thesis, Duke University. Retrieved from https://hdl.handle.net/10161/5541.
Collections
  • Masters Theses
More Info
Show full item record
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

Rights for Collection: Masters Theses


Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info

Make Your Work Available Here

How to Deposit

Browse

All of DukeSpaceCommunities & CollectionsAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit DateThis CollectionAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit Date

My Account

LoginRegister

Statistics

View Usage Statistics
Duke University Libraries

Contact Us

411 Chapel Drive
Durham, NC 27708
(919) 660-5870
Perkins Library Service Desk

Digital Repositories at Duke

  • Report a problem with the repositories
  • About digital repositories at Duke
  • Accessibility Policy
  • Deaccession and DMCA Takedown Policy

TwitterFacebookYouTubeFlickrInstagramBlogs

Sign Up for Our Newsletter
  • Re-use & Attribution / Privacy
  • Harmful Language Statement
  • Support the Libraries
Duke University