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Investigations of Inositol Phosphate-Mediated Transcription

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Date
2012
Author
Hatch, Ace Joseph
Advisor
York, John D
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Abstract

Inositol phosphates (IPs) are eukaryotic signaling molecules that play important roles in a wide range of biological processes. IPs are required for embryonic development and patterning, insulin secretion, the regulation of telomere length, proper progression through the cell cycle, and the regulation of ion channels. This work uses the yeast Saccharomyces cerevisiae as a model system for investigating the functions of IPs and focuses on the transcriptional regulation of the gene encoding the secreted mating pheromone MFα2 by the IP kinase Ipk2 (also called Arg82, ArgR3, and IPMK). This work shows that Ipk2 has both kinase-dependent and kinase-independent functions in regulating the transcription of MFα2. Transcription of MFα2 is also dependent upon the integrity of an Mcm1-binding site in its promoter. This is the first description of a role for this binding site in the transcription of MFα2.

<italic>In vivo</italic> and <italic>in vitro</italic> screening approaches to identify additional factors associated with MF&alpha;2 expression or with IP biology generally are also described. These unbiased approaches provide some valuable insight for further investigations.

Type
Dissertation
Department
Biochemistry
Subject
Biochemistry
Inositol
Phosphate
Transcription
Yeast
Permalink
https://hdl.handle.net/10161/5777
Citation
Hatch, Ace Joseph (2012). Investigations of Inositol Phosphate-Mediated Transcription. Dissertation, Duke University. Retrieved from https://hdl.handle.net/10161/5777.
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