Show simple item record Olson, JC Cuff, CF Lukomski, S Lukomska, E Canizales, Y Wu, B Crout, RJ Thomas, JG McNeil, DW Weyant, RJ Marazita, ML Paster, BJ Elliott, T
dc.coverage.spatial England 2012-09-17T18:41:37Z 2011-03-01
dc.identifier 1472-6831-11-7
dc.identifier.citation BMC Oral Health, 2011, 11 pp. 7 - ?
dc.description.abstract BACKGROUND: West Virginia has the worst oral health in the United States, but the reasons for this are unclear. This pilot study explored the etiology of this disparity using culture-independent analyses to identify bacterial species associated with oral disease. METHODS: Bacteria in subgingival plaque samples from twelve participants in two independent West Virginia dental-related studies were characterized using 16S rRNA gene sequencing and Human Oral Microbe Identification Microarray (HOMIM) analysis. Unifrac analysis was used to characterize phylogenetic differences between bacterial communities obtained from plaque of participants with low or high oral disease, which was further evaluated using clustering and Principal Coordinate Analysis. RESULTS: Statistically different bacterial signatures (P<0.001) were identified in subgingival plaque of individuals with low or high oral disease in West Virginia based on 16S rRNA gene sequencing. Low disease contained a high frequency of Veillonella and Streptococcus, with a moderate number of Capnocytophaga. High disease exhibited substantially increased bacterial diversity and included a large proportion of Clostridiales cluster bacteria (Selenomonas, Eubacterium, Dialister). Phylogenetic trees constructed using 16S rRNA gene sequencing revealed that Clostridiales were repeated colonizers in plaque associated with high oral disease, providing evidence that the oral environment is somehow influencing the bacterial signature linked to disease. CONCLUSIONS: Culture-independent analyses identified an atypical bacterial signature associated with high oral disease in West Virginians and provided evidence that the oral environment influenced this signature. Both findings provide insight into the etiology of the oral disparity in West Virginia.
dc.format.extent 7 - ?
dc.language eng
dc.relation.ispartof BMC Oral Health
dc.relation.isversionof 10.1186/1472-6831-11-7
dc.subject Adult
dc.subject Aged
dc.subject Aged, 80 and over
dc.subject Bacterial Typing Techniques
dc.subject Cluster Analysis
dc.subject DNA, Bacterial
dc.subject Dental Plaque
dc.subject Humans
dc.subject Middle Aged
dc.subject Mouth Diseases
dc.subject Oligonucleotide Array Sequence Analysis
dc.subject Phylogeny
dc.subject Pilot Projects
dc.subject Principal Component Analysis
dc.subject RNA, Ribosomal, 16S
dc.subject Tooth Diseases
dc.subject West Virginia
dc.subject Young Adult
dc.title Use of 16S ribosomal RNA gene analyses to characterize the bacterial signature associated with poor oral health in West Virginia.
dc.type Journal Article
duke.description.issue 1 en_US
duke.description.startpage 1 en_US
duke.description.volume 11 en_US
dc.relation.journal BMC Oral Health en_US
pubs.organisational-group /Duke
pubs.organisational-group /Duke/Institutes and Provost's Academic Units
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers
pubs.organisational-group /Duke/Institutes and Provost's Academic Units/University Institutes and Centers/Global Health Institute
pubs.organisational-group /Duke/School of Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine
pubs.organisational-group /Duke/School of Medicine/Clinical Science Departments/Medicine/Medicine, Geriatrics
pubs.organisational-group /Duke/School of Nursing
pubs.publication-status Published online
pubs.volume 11
dc.identifier.eissn 1472-6831

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