β-Adrenergic Receptor Kinase-1 Levels in Catecholamine-Induced Myocardial Hypertrophy
Abstract
Pressure overload ventricular hypertrophy is accompanied by dysfunctional β-adrenergic
receptor signaling due to increased levels of the β-adrenergic receptor kinase-1,
which phosphorylates and desensitizes β-adrenergic receptors. In this study, we examined
whether increased β-adrenergic receptor kinase 1 expression is associated with myocardial
hypertrophy induced by adrenergic stimulation. With use of implanted mini-osmotic
pumps, we treated mice with isoproterenol, phenylephrine, or vehicle to distinguish
between α1- and β-adrenergic stimulation. Both treatments resulted in cardiac hypertrophy,
but only isoproterenol induced significant increases in β-adrenergic receptor kinase-1
protein levels and activity. Similarly, in isolated adult rat cardiac myocytes, 24
hours of isoproterenol stimulation resulted in a significant 2.8-fold increase in
β-adrenergic receptor kinase-1 protein levels, whereas 24 hours of phenylephrine treatment
did not alter β-adrenergic receptor kinase-1 expression. Our results indicate that
increased β-adrenergic receptor kinase-1 is not invariably associated with myocardial
hypertrophy but apparently is controlled by the state of β-adrenergic receptor activation.
Type
Journal articleSubject
myocardial hypertrophyβ-adrenergic receptor
G protein–coupled receptor kinases
desensitization
catecholamines
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https://hdl.handle.net/10161/5937Published Version (Please cite this version)
10.1161/01.HYP.33.1.396Citation
Iaccarino, G., P. C. Dolber, et al. (1999). "β-Adrenergic Receptor Kinase-1 Levels
in Catecholamine-Induced Myocardial Hypertrophy." Hypertension 33(1): 396-401.
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Robert J. Lefkowitz
The Chancellor's Distinguished Professor of Medicine
Dr. Lefkowitz’s memoir, A Funny Thing Happened on the Way to Stockholm, recounts his
early career as a cardiologist and his transition to biochemistry, which led to his
Nobel Prize win.
Robert J. Lefkowitz, M.D. is James B. Duke Professor of Medicine and Professor of
Biochemistry and Chemistry at the Duke University Medical Center. He has been an Investigator
of the

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