Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer in Tanzanian women.
Abstract
BACKGROUND: Infection with human papillomavirus (HPV) is associated with uterine cervical
intraepithelial neoplasia (CIN) and invasive cancers (ICC). Approximately 80% of ICC
cases are diagnosed in under-developed countries. Vaccine development relies on knowledge
of HPV genotypes characteristic of LSIL, HSIL and cancer; however, these genotypes
remain poorly characterized in many African countries. To contribute to the characterization
of HPV genotypes in Northeastern Tanzania, we recruited 215 women from the Reproductive
Health Clinic at Kilimanjaro Christian Medical Centre. Cervical scrapes and biopsies
were obtained for cytology and HPV DNA detection. RESULTS: 79 out of 215 (36.7%) enrolled
participants tested positive for HPV DNA, with a large proportion being multiple infections
(74%). The prevalence of HPV infection increased with lesion grade (14% in controls,
67% in CIN1 cases and 88% in CIN2-3). Among ICC cases, 89% had detectable HPV. Overall,
31 HPV genotypes were detected; the three most common HPV genotypes among ICC were
HPV16, 35 and 45. In addition to these genotypes, co-infection with HPV18, 31, 33,
52, 58, 68 and 82 was found in 91% of ICC. Among women with CIN2-3, HPV53, 58 and
84/83 were the most common. HPV35, 45, 53/58/59 were the most common among CIN1 cases.
CONCLUSIONS: In women with no evidence of cytological abnormalities, the most prevalent
genotypes were HPV58 with HPV16, 35, 52, 66 and 73 occurring equally. Although numerical
constraints limit inference, findings that 91% of ICC harbor only a small number of
HPV genotypes suggests that prevention efforts including vaccine development or adjuvant
screening should focus on these genotypes.
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https://hdl.handle.net/10161/5939Published Version (Please cite this version)
10.1186/1750-9378-6-20Publication Info
Vidal, Adriana C; Murphy, Susan K; Hernandez, Brenda Y; Vasquez, Brandi; Bartlett,
John A; Oneko, Olola; ... Hoyo, Cathrine (2011). Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer
in Tanzanian women. Infect Agent Cancer, 6(1). pp. 20. 10.1186/1750-9378-6-20. Retrieved from https://hdl.handle.net/10161/5939.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
John Alexander Bartlett
Professor of Medicine
My clinical investigation is focused on the pathogenesis and treatment of HIV infection
and its complicastions, especially in resource-limited settings. Key Words: HIV infection,
AIDS, treatment strategies, treatment failure, co-infections, resource-limited settings
Susan Kay Murphy
Associate Professor in Obstetrics and Gynecology
My research interests are largely centered around epigenetics and the role of epigenetic
modifications in health and disease. My research projects include studies of gynecologic
malignancies, including working on approaches to target ovarian cancer cells that
survive chemotherapy and later give rise to recurrent disease. I have ongoing collaborative
projects in which we investigate the nature of the Developmental Origins of Health
and Disease (DOHaD) hypothesis. DOHaD reflects the ide
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