Instrument independent diffuse reflectance spectroscopy.
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Diffuse reflectance spectroscopy with a fiber optic probe is a powerful tool for quantitative tissue characterization and disease diagnosis. Significant systematic errors can arise in the measured reflectance spectra and thus in the derived tissue physiological and morphological parameters due to real-time instrument fluctuations. We demonstrate a novel fiber optic probe with real-time, self-calibration capability that can be used for UV-visible diffuse reflectance spectroscopy in biological tissue in clinical settings. The probe is tested in a number of synthetic liquid phantoms over a wide range of tissue optical properties for significant variations in source intensity fluctuations caused by instrument warm up and day-to-day drift. While the accuracy for extraction of absorber concentrations is comparable to that achieved with the traditional calibration (with a reflectance standard), the accuracy for extraction of reduced scattering coefficients is significantly improved with the self-calibration probe compared to traditional calibration. This technology could be used to achieve instrument-independent diffuse reflectance spectroscopy in vivo and obviate the need for instrument warm up and post∕premeasurement calibration, thus saving up to an hour of precious clinical time.
Equipment Failure Analysis
Fiber Optic Technology
Pattern Recognition, Automated
Reproducibility of Results
Sensitivity and Specificity
Published Version (Please cite this version)10.1117/1.3524303
Publication InfoFu, HL; Ramanujam, Nimmi; & Yu, Bing (2011). Instrument independent diffuse reflectance spectroscopy. J Biomed Opt, 16(1). pp. 011010. 10.1117/1.3524303. Retrieved from https://hdl.handle.net/10161/5977.
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Robert W. Carr, Jr., Distinguished Professor of Biomedical Engineering
Dr. Ramanujam is an innovator, educator and entrepreneur. Her mission is to develop technology that will have a wide reaching impact on women's health. She directs the center for Global Women’s Health Technologies at Duke where she empowers trainees at Duke and beyond to create impactful solutions to improve the lives of women and girls globally. Dr. Ramanujam has spent the last two decades developing pr