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Instrument independent diffuse reflectance spectroscopy.

dc.contributor.author Yu, Bing
dc.contributor.author Fu, Henry L
dc.contributor.author Ramanujam, Nirmala
dc.coverage.spatial United States
dc.date.accessioned 2012-11-01T20:16:31Z
dc.date.issued 2011-01
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/21280897
dc.identifier.uri https://hdl.handle.net/10161/5977
dc.description.abstract Diffuse reflectance spectroscopy with a fiber optic probe is a powerful tool for quantitative tissue characterization and disease diagnosis. Significant systematic errors can arise in the measured reflectance spectra and thus in the derived tissue physiological and morphological parameters due to real-time instrument fluctuations. We demonstrate a novel fiber optic probe with real-time, self-calibration capability that can be used for UV-visible diffuse reflectance spectroscopy in biological tissue in clinical settings. The probe is tested in a number of synthetic liquid phantoms over a wide range of tissue optical properties for significant variations in source intensity fluctuations caused by instrument warm up and day-to-day drift. While the accuracy for extraction of absorber concentrations is comparable to that achieved with the traditional calibration (with a reflectance standard), the accuracy for extraction of reduced scattering coefficients is significantly improved with the self-calibration probe compared to traditional calibration. This technology could be used to achieve instrument-independent diffuse reflectance spectroscopy in vivo and obviate the need for instrument warm up and post∕premeasurement calibration, thus saving up to an hour of precious clinical time.
dc.language eng
dc.publisher SPIE-Intl Soc Optical Eng
dc.relation.ispartof J Biomed Opt
dc.relation.isversionof 10.1117/1.3524303
dc.subject Computer-Aided Design
dc.subject Equipment Design
dc.subject Equipment Failure Analysis
dc.subject Fiber Optic Technology
dc.subject Lighting
dc.subject Pattern Recognition, Automated
dc.subject Photometry
dc.subject Reproducibility of Results
dc.subject Sensitivity and Specificity
dc.subject Spectrophotometry, Ultraviolet
dc.title Instrument independent diffuse reflectance spectroscopy.
dc.type Journal article
duke.contributor.id Yu, Bing|0373220
duke.description.issue 1
duke.description.volume 16
dc.relation.journal Journal of Biomedical Optics
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/21280897
pubs.begin-page 011010
pubs.issue 1
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Science & Society
pubs.organisational-group Global Health Institute
pubs.organisational-group Initiatives
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Pharmacology & Cancer Biology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 16
dc.identifier.eissn 1560-2281


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