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Urban Small Mammal Pathogen Dynamics in Singapore - Prevalence, Distribution, and Genetic Diversity

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Date
2012
Author
Garrett, Ryan Christopher
Advisor
Smith, Gavin
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Abstract

The goals of this thesis are to identify pathogens that are present in the small mammal populations in urban environment of Singapore, the distribution of these pathogens throughout the island, and to the assess the genetic diversity and phylogenetic relationships of these pathogens. These three components of data collection will be triangulated to assess the risk to human populations from zoonotic diseases. Small mammals were collected throughout a total of ten trapping locations over the period of three months. Rodents were euthanized according to guidelines specified by National Environmental Agency of Singapore. Organs and blood samples were harvested from collected rodents in order to extract DNA and RNA used in pathogen detection. PCR detection revealed several positive results for the presence of pathogens including Hantavirus, Trypanosoma, Rickettsia, Leptospirosis, Borrelia, and Anaplasmsa. These diseases are known to infect humans and may pose a threat to the public health of the Singaporean population.

Type
Master's thesis
Department
Global Health
Subject
Public health
Molecular biology
Ecology
Emerging Infectious Diseases
Hantavirus
Pathogen dynamics
Rodent
Singapore
Suncus murinus
Permalink
https://hdl.handle.net/10161/6198
Citation
Garrett, Ryan Christopher (2012). Urban Small Mammal Pathogen Dynamics in Singapore - Prevalence, Distribution, and Genetic Diversity. Master's thesis, Duke University. Retrieved from https://hdl.handle.net/10161/6198.
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This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 United States License.

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