Portable, Fiber-Based, Diffuse Reflection Spectroscopy (DRS) Systems for Estimating Tissue Optical Properties.
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Steady-state diffuse reflection spectroscopy is a well-studied optical technique that can provide a noninvasive and quantitative method for characterizing the absorption and scattering properties of biological tissues. Here, we compare three fiber-based diffuse reflection spectroscopy systems that were assembled to create a light-weight, portable, and robust optical spectrometer that could be easily translated for repeated and reliable use in mobile settings. The three systems were built using a broadband light source and a compact, commercially available spectrograph. We tested two different light sources and two spectrographs (manufactured by two different vendors). The assembled systems were characterized by their signal-to-noise ratios, the source-intensity drifts, and detector linearity. We quantified the performance of these instruments in extracting optical properties from diffuse reflectance spectra in tissue-mimicking liquid phantoms with well-controlled optical absorption and scattering coefficients. We show that all assembled systems were able to extract the optical absorption and scattering properties with errors less than 10%, while providing greater than ten-fold decrease in footprint and cost (relative to a previously well-characterized and widely used commercial system). Finally, we demonstrate the use of these small systems to measure optical biomarkers in vivo in a small-animal model cancer therapy study. We show that optical measurements from the simple portable system provide estimates of tumor oxygen saturation similar to those detected using the commercial system in murine tumor models of head and neck cancer.
Published Version (Please cite this version)10.1366/10-06052
Publication InfoChang, K; Chang, Vivide T; Deng, YF; Klein, D; Phelps, JE; Ramanujam, Nimmi; & Vishwanath, K (2011). Portable, Fiber-Based, Diffuse Reflection Spectroscopy (DRS) Systems for Estimating Tissue Optical Properties. Appl Spectrosc, 62(2). pp. 206-215. 10.1366/10-06052. Retrieved from http://hdl.handle.net/10161/6344.
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Robert W. Carr, Jr., Professor of Biomedical Engineering
Dr. Ramanujam is the Robert W. Carr Professor of Biomedical Engineering, and also a faculty member in the Global Health Institute and Dept. Pharmacology and Cell Biology at Duke University. She is an innovator, educator and entrepreneur and her mission is to develop and leverage technology to have the most wide reaching impact in women’s health. She directs the center for Global Women’s Health Technologies (GWHT), a partnership between the Pratt School of Engineering and the Duke