SYSTEMATIC OMICS ANALYSIS REVIEW TOOL TO SUPPORT RISK ASSESSMENT
Abstract
Environmental health risk assessors are challenged to understand and incorporate new
data streams as the field of toxicology continues to adopt new molecular and systems
biology technologies. Systematic screening reviews can help risk assessors determine
which studies to consider for inclusion in a human health assessment. A tool for systematic
reviews should be standardized and transparent in order to consistently determine
which studies meet minimum quality criteria prior to performing in-depth analyses
of the data. The Systematic Omics Analysis Review (SOAR) tool is a spreadsheet of
35 objective questions developed by domain experts, focused on transcriptomic microarray
studies, and including four main topics: test system, test substance, experimental
design, and microarray data. The tool will be used as a guide to identify studies
that meet basic published quality criteria, such as those defined by the Minimum Information
About a Microarray Experiment (MIAME) standard and the Toxicological data Reliability
assessment Tool (ToxR Tool). Seven scientists were recruited to test the tool by using
it to rate 19 published manuscripts that study chemical exposures with microarrays.
Using their feedback, questions were weighted based on importance of the information
and a suitability cutoff was set for each of the four topic sections. The final validation
resulted in 100% agreement between the users on four separate manuscripts, showing
that the SOAR tool may be used to facilitate the standardized and transparent screening
of microarray literature for environmental human health risk assessment.
Type
Master's projectPermalink
https://hdl.handle.net/10161/6699Citation
McConnell, Emma R. (2013). SYSTEMATIC OMICS ANALYSIS REVIEW TOOL TO SUPPORT RISK ASSESSMENT. Master's project, Duke University. Retrieved from https://hdl.handle.net/10161/6699.Collections
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