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A beta-adrenergic receptor kinase-like enzyme is involved in olfactory signal termination.

dc.contributor.author Schleicher, S
dc.contributor.author Boekhoff, I
dc.contributor.author Arriza, J
dc.contributor.author Lefkowitz, RJ
dc.contributor.author Breer, H
dc.coverage.spatial United States
dc.date.accessioned 2013-09-10T17:14:35Z
dc.date.issued 1993-02-15
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/8381966
dc.identifier.issn 0027-8424
dc.identifier.uri https://hdl.handle.net/10161/7848
dc.description.abstract We have previously shown that second-messenger-dependent kinases (cAMP-dependent kinase, protein kinase C) in the olfactory system are essential in terminating second-messenger signaling in response to odorants. We now document that subtype 2 of the beta-adrenergic receptor kinase (beta ARK) is also involved in this process. By using subtype-specific antibodies to beta ARK-1 and beta ARK-2, we show that beta ARK-2 is preferentially expressed in the olfactory epithelium in contrast to findings in most other tissues. Heparin, an inhibitor of beta ARK, as well as anti-beta ARK-2 antibodies, (i) completely prevents the rapid decline of second-messenger signals (desensitization) that follows odorant stimulation and (ii) strongly inhibits odorant-induced phosphorylation of olfactory ciliary proteins. In contrast, beta ARK-1 antibodies are without effect. Inhibitors of protein kinase A and protein kinase C also block odorant-induced desensitization and phosphorylation. These data suggest that a sequential interplay of second-messenger-dependent and receptor-specific kinases is functionally involved in olfactory desensitization.
dc.language eng
dc.publisher Proceedings of the National Academy of Sciences
dc.relation.ispartof Proc Natl Acad Sci U S A
dc.subject Animals
dc.subject Antibodies
dc.subject Cerebral Cortex
dc.subject Chemoreceptor Cells
dc.subject Cilia
dc.subject Cyclic AMP-Dependent Protein Kinases
dc.subject Epithelium
dc.subject Heparin
dc.subject Immune Sera
dc.subject Kinetics
dc.subject Odorants
dc.subject Olfactory Pathways
dc.subject Organ Specificity
dc.subject Phosphorylation
dc.subject Protein Kinase Inhibitors
dc.subject Protein Kinases
dc.subject Rats
dc.subject Rats, Sprague-Dawley
dc.subject Receptors, Adrenergic, beta
dc.subject Signal Transduction
dc.subject Smell
dc.subject Time Factors
dc.subject beta-Adrenergic Receptor Kinases
dc.title A beta-adrenergic receptor kinase-like enzyme is involved in olfactory signal termination.
dc.type Journal article
duke.contributor.id Lefkowitz, RJ|0096962
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/8381966
pubs.begin-page 1420
pubs.end-page 1424
pubs.issue 4
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biochemistry
pubs.organisational-group Chemistry
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Pathology
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.publication-status Published
pubs.volume 90


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