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Expansion of the alpha 2-adrenergic receptor family: cloning and characterization of a human alpha 2-adrenergic receptor subtype, the gene for which is located on chromosome 2.

dc.contributor.author Allen, LF
dc.contributor.author Caron, MG
dc.contributor.author King, K
dc.contributor.author Lefkowitz, Robert J
dc.contributor.author Lomasney, JW
dc.contributor.author Lorenz, W
dc.contributor.author Regan, JW
dc.contributor.author Yang-Feng, TL
dc.coverage.spatial United States
dc.date.accessioned 2013-09-24T17:14:51Z
dc.date.issued 1990-07
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/2164221
dc.identifier.issn 0027-8424
dc.identifier.uri https://hdl.handle.net/10161/7862
dc.description.abstract Pharmacologic, biochemical, and genetic analyses have demonstrated the existence of multiple alpha 2-adrenergic receptor (alpha 2AR) subtypes. We have cloned a human alpha 2AR by using the polymerase chain reaction with oligonucleotide primers homologous to conserved regions of the previously cloned alpha 2ARs, the genes for which are located on human chromosomes 4 (C4) and 10 (C10). The deduced amino acid sequence encodes a protein of 450 amino acids whose putative topology is similar to that of the family of guanine nucleotide-binding protein-coupled receptors, but whose structure most closely resembles that of the alpha 2ARs. Competition curve analysis of the binding properties of the receptor expressed in COS-7 cells with a variety of adrenergic ligands demonstrates a unique alpha 2AR pharmacology. Hybridization with somatic cell hybrids shows that the gene for this receptor is located on chromosome 2. Northern blot analysis of various rat tissues shows expression in liver and kidney. The unique pharmacology and tissue localization of this receptor suggest that this is an alpha 2AR subtype not previously identified by classical pharmacological or ligand binding approaches.
dc.language eng
dc.relation.ispartof Proc Natl Acad Sci U S A
dc.subject Amino Acid Sequence
dc.subject Animals
dc.subject Base Sequence
dc.subject Binding, Competitive
dc.subject Cell Line
dc.subject Chromosomes, Human, Pair 2
dc.subject Cloning, Molecular
dc.subject Codon
dc.subject Female
dc.subject Genomic Library
dc.subject Humans
dc.subject Ligands
dc.subject Molecular Sequence Data
dc.subject Multigene Family
dc.subject Oligonucleotide Probes
dc.subject Placenta
dc.subject Polymerase Chain Reaction
dc.subject Pregnancy
dc.subject Receptors, Adrenergic, alpha
dc.subject Sequence Homology, Nucleic Acid
dc.subject Transfection
dc.title Expansion of the alpha 2-adrenergic receptor family: cloning and characterization of a human alpha 2-adrenergic receptor subtype, the gene for which is located on chromosome 2.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/2164221
pubs.begin-page 5094
pubs.end-page 5098
pubs.issue 13
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biochemistry
pubs.organisational-group Cell Biology
pubs.organisational-group Chemistry
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Neurobiology
pubs.organisational-group Pathology
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 87


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