Show simple item record

Chromosomal organization of adrenergic receptor genes.

dc.contributor.author Caron, MG
dc.contributor.author Cotecchia, S
dc.contributor.author Francke, U
dc.contributor.author Frielle, T
dc.contributor.author Lefkowitz, Robert J
dc.contributor.author Xue, FY
dc.contributor.author Yang-Feng, TL
dc.contributor.author Zhong, WW
dc.coverage.spatial United States
dc.date.accessioned 2013-09-24T17:29:19Z
dc.date.issued 1990-02
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/2154750
dc.identifier.issn 0027-8424
dc.identifier.uri https://hdl.handle.net/10161/7866
dc.description.abstract The adrenergic receptors (ARs) (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. We have previously assigned the genes for beta 2- and alpha 2-AR to human chromosomes 5 and 10, respectively. By Southern analysis of somatic cell hybrids and in situ chromosomal hybridization, we have now mapped the alpha 1-AR gene to chromosome 5q32----q34, the same position as beta 2-AR, and the beta 1-AR gene to chromosome 10q24----q26, the region where alpha 2-AR is located. In mouse, both alpha 2- and beta 1-AR genes were assigned to chromosome 19, and the alpha 1-AR locus was localized to chromosome 11. Pulsed field gel electrophoresis has shown that the alpha 1- and beta 2-AR genes in humans are within 300 kilobases (kb) and the distance between the alpha 2- and beta 1-AR genes is less than 225 kb. The proximity of these two pairs of AR genes and the sequence similarity that exists among all the ARs strongly suggest that they are evolutionarily related. Moreover, they likely arose from a common ancestral receptor gene and subsequently diverged through gene duplication and chromosomal duplication to perform their distinctive roles in mediating the physiological effects of catecholamines. The AR genes thus provide a paradigm for understanding the evolution of such structurally conserved yet functionally divergent families of receptor molecules.
dc.language eng
dc.relation.ispartof Proc Natl Acad Sci U S A
dc.subject Animals
dc.subject Biological Evolution
dc.subject Blotting, Southern
dc.subject Cell Line
dc.subject Cells, Cultured
dc.subject Chromosome Banding
dc.subject Chromosome Mapping
dc.subject Chromosomes, Human
dc.subject Cricetinae
dc.subject Cricetulus
dc.subject DNA
dc.subject Fibroblasts
dc.subject Humans
dc.subject Hybrid Cells
dc.subject Lymphocytes
dc.subject Mice
dc.subject Nucleic Acid Hybridization
dc.subject Receptors, Adrenergic, alpha
dc.subject Receptors, Adrenergic, beta
dc.title Chromosomal organization of adrenergic receptor genes.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/2154750
pubs.begin-page 1516
pubs.end-page 1520
pubs.issue 4
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biochemistry
pubs.organisational-group Cell Biology
pubs.organisational-group Chemistry
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Neurobiology
pubs.organisational-group Pathology
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 87


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record