Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.
Abstract
The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides
corresponding to the partial amino acid sequence of the receptor protein purified
from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue
polypeptide that shows the most sequence identity with the other adrenergic receptors
and the putative protein product of the related clone G-21. Similarities with the
muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells
confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol
phospholipid metabolism.
Type
Journal articleSubject
AnimalsBase Sequence
Cloning, Molecular
Cricetinae
DNA
GTP-Binding Proteins
Gene Expression Regulation
Molecular Sequence Data
Phosphatidylinositols
Receptors, Adrenergic, alpha
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Marc G. Caron
James B. Duke Distinguished Professor of Cell Biology
Studies of the mechanisms of action and regulation of hormones and neurotransmitters
at the cellular and molecular levels constitute the main goals our of research activities.
G protein-coupled receptors (GPCR) mediate the actions of signaling molecules from
unicellular organisms to man. We have used adrenergic and dopamine receptors to characterize
the structure/function and regulation mechanisms of these prototypes of G protein-coupled
receptors. Another approach has been to characterize
Robert J. Lefkowitz
James B. Duke Distinguished Professor of Medicine
The focus of work in this laboratory is on the elucidation of the molecular properties
and regulatory mechanisms controlling the function of G protein-coupled receptors.
As model systems we utilize the so called adrenergic receptors for adrenaline and
related molecules. The goal is to learn the general principles of signal transduction
from the outside to the inside of the cell which are involved in systems as diverse
as sensory perception, neuro- transmitter and hormonal signaling. Stud
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