Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.
Abstract
The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides
corresponding to the partial amino acid sequence of the receptor protein purified
from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue
polypeptide that shows the most sequence identity with the other adrenergic receptors
and the putative protein product of the related clone G-21. Similarities with the
muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells
confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol
phospholipid metabolism.
Type
Journal articleSubject
AnimalsBase Sequence
Cloning, Molecular
Cricetinae
DNA
GTP-Binding Proteins
Gene Expression Regulation
Molecular Sequence Data
Phosphatidylinositols
Receptors, Adrenergic, alpha
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Marc G. Caron
James B. Duke Distinguished Professor of Cell Biology
Studies of the mechanisms of action and regulation of hormones and neurotransmitters
at the cellular and molecular levels constitute the main goals our of research activities.
G protein-coupled receptors (GPCR) mediate the actions of signaling molecules from
unicellular organisms to man. We have used adrenergic and dopamine receptors to characterize
the structure/function and regulation mechanisms of these prototypes of G protein-coupled
receptors. Another approach has been to characterize
This author no longer has a Scholars@Duke profile, so the information shown here reflects
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Robert J. Lefkowitz
James B. Duke Distinguished Professor of Medicine
Dr. Lefkowitz’s memoir, A Funny Thing Happened on the Way to Stockholm, recounts his
early career as a cardiologist and his transition to biochemistry, which led to his
Nobel Prize win.
Robert J. Lefkowitz, M.D. is James B. Duke Professor of Medicine and Professor of
Biochemistry and Chemistry at the Duke University Medical Center. He has been an Investigator
of the
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