Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.
Abstract
An alpha 2-adrenergic receptor subtype has been cloned from a human kidney cDNA library
using the gene for the human platelet alpha 2-adrenergic receptor as a probe. The
deduced amino acid sequence resembles the human platelet alpha 2-adrenergic receptor
and is consistent with the structure of other members of the family of guanine nucleotide-binding
protein-coupled receptors. The cDNA was expressed in a mammalian cell line (COS-7),
and the alpha 2-adrenergic ligand [3H]rauwolscine was bound. Competition curve analysis
with a variety of adrenergic ligands suggests that this cDNA clone represents the
alpha 2B-adrenergic receptor. The gene for this receptor is on human chromosome 4,
whereas the gene for the human platelet alpha 2-adrenergic receptor (alpha 2A) lies
on chromosome 10. This ability to express the receptor in mammalian cells, free of
other adrenergic receptor subtypes, should help in developing more selective alpha-adrenergic
ligands.
Type
Journal articleSubject
Amino Acid SequenceAnimals
Base Sequence
Cell Line
Chromosomes, Human, Pair 4
Cloning, Molecular
DNA
Genes
Humans
Kidney
Kinetics
Models, Molecular
Molecular Sequence Data
Protein Conformation
Receptors, Adrenergic, alpha
Transcription, Genetic
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Marc G. Caron
James B. Duke Distinguished Professor of Cell Biology
Studies of the mechanisms of action and regulation of hormones and neurotransmitters
at the cellular and molecular levels constitute the main goals our of research activities.
G protein-coupled receptors (GPCR) mediate the actions of signaling molecules from
unicellular organisms to man. We have used adrenergic and dopamine receptors to characterize
the structure/function and regulation mechanisms of these prototypes of G protein-coupled
receptors. Another approach has been to characterize
Robert J. Lefkowitz
James B. Duke Distinguished Professor of Medicine
Dr. Lefkowitz’s memoir, A Funny Thing Happened on the Way to Stockholm, recounts his
early career as a cardiologist and his transition to biochemistry, which led to his
Nobel Prize win.
Robert J. Lefkowitz, M.D. is James B. Duke Professor of Medicine and Professor of
Biochemistry and Chemistry at the Duke University Medical Center. He has been an Investigator
of the
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