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Cloning of the cDNA for the human beta 1-adrenergic receptor.

dc.contributor.author Caron, MG
dc.contributor.author Collins, Sheila
dc.contributor.author Daniel, KW
dc.contributor.author Frielle, T
dc.contributor.author Kobilka, BK
dc.contributor.author Lefkowitz, Robert J
dc.coverage.spatial United States
dc.date.accessioned 2013-09-24T17:58:39Z
dc.date.issued 1987-11
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/2825170
dc.identifier.issn 0027-8424
dc.identifier.uri https://hdl.handle.net/10161/7875
dc.description.abstract Screening of a human placenta lambda gt11 library has led to the isolation of the cDNA for the human beta 1-adrenergic receptor (beta 1AR). Used as the probe was the human genomic clone termed G-21. This clone, which contains an intronless gene for a putative receptor, was previously isolated by virtue of its cross hybridization with the human beta 2-adrenergic receptor (beta 2AR). The 2.4-kilobase cDNA for the human beta 1AR encodes a protein of 477 amino acid residues that is 69% homologous with the avian beta AR but only 54% homologous with the human beta 2AR. This suggests that the avian gene encoding beta AR and the human gene encoding beta 1AR evolved from a common ancestral gene. RNA blot analysis indicates a message of 2.5 kilobases in rat tissues, with a pattern of tissue distribution consistent with beta 1AR binding. This pattern is quite distinct from the pattern obtained when the beta 2AR cDNA is used as a probe. Expression of receptor protein in Xenopus laevis oocytes conveys adenylate cyclase responsiveness to catecholamines with a typical beta 1AR specificity. This contrasts with the typical beta 2 subtype specificity observed when the human beta 2AR cDNA is expressed in this system. Mammalian beta 1AR and beta 2AR are thus products of distinct genes, both of which are apparently related to the putative G-21 receptor.
dc.language eng
dc.relation.ispartof Proc Natl Acad Sci U S A
dc.subject Amino Acid Sequence
dc.subject Animals
dc.subject Base Sequence
dc.subject DNA
dc.subject DNA, Recombinant
dc.subject Humans
dc.subject Molecular Sequence Data
dc.subject Receptors, Adrenergic, beta
dc.subject Sequence Homology, Nucleic Acid
dc.subject Turkeys
dc.title Cloning of the cDNA for the human beta 1-adrenergic receptor.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/2825170
pubs.begin-page 7920
pubs.end-page 7924
pubs.issue 22
pubs.organisational-group Basic Science Departments
pubs.organisational-group Biochemistry
pubs.organisational-group Cell Biology
pubs.organisational-group Chemistry
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Medicine, Endocrinology, Metabolism, and Nutrition
pubs.organisational-group Neurobiology
pubs.organisational-group Pathology
pubs.organisational-group Psychiatry & Behavioral Sciences
pubs.organisational-group Psychiatry & Behavioral Sciences, General Psychiatry
pubs.organisational-group School of Medicine
pubs.organisational-group Trinity College of Arts & Sciences
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published
pubs.volume 84


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