cDNA for the human beta 2-adrenergic receptor: a protein with multiple membrane-spanning domains and encoded by a gene whose chromosomal location is shared with that of the receptor for platelet-derived growth factor.
Abstract
We have isolated and sequenced a cDNA encoding the human beta 2-adrenergic receptor.
The deduced amino acid sequence (413 residues) is that of a protein containing seven
clusters of hydrophobic amino acids suggestive of membrane-spanning domains. While
the protein is 87% identical overall with the previously cloned hamster beta 2-adrenergic
receptor, the most highly conserved regions are the putative transmembrane helices
(95% identical) and cytoplasmic loops (93% identical), suggesting that these regions
of the molecule harbor important functional domains. Several of the transmembrane
helices also share lesser degrees of identity with comparable regions of select members
of the opsin family of visual pigments. We have localized the gene for the beta 2-adrenergic
receptor to q31-q32 on chromosome 5. This is the same position recently determined
for the gene encoding the receptor for platelet-derived growth factor and is adjacent
to that for the FMS protooncogene, which encodes the receptor for the macrophage colony-stimulating
factor.
Type
Journal articleSubject
Amino Acid SequenceAnimals
Base Sequence
Cell Membrane
Chromosome Mapping
Chromosomes, Human, Pair 14
Cricetinae
Cricetulus
DNA
Female
Humans
Hybrid Cells
Placenta
Platelet-Derived Growth Factor
Pregnancy
Protein Conformation
Receptors, Adrenergic, beta
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Marc G. Caron
James B. Duke Distinguished Professor of Cell Biology
Studies of the mechanisms of action and regulation of hormones and neurotransmitters
at the cellular and molecular levels constitute the main goals our of research activities.
G protein-coupled receptors (GPCR) mediate the actions of signaling molecules from
unicellular organisms to man. We have used adrenergic and dopamine receptors to characterize
the structure/function and regulation mechanisms of these prototypes of G protein-coupled
receptors. Another approach has been to characterize
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Robert J. Lefkowitz
The Chancellor's Distinguished Professor of Medicine
Dr. Lefkowitz’s memoir, A Funny Thing Happened on the Way to Stockholm, recounts his
early career as a cardiologist and his transition to biochemistry, which led to his
Nobel Prize win.
Robert J. Lefkowitz, M.D. is James B. Duke Professor of Medicine and Professor of
Biochemistry and Chemistry at the Duke University Medical Center. He has been an Investigator
of the
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