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In Vivo luminescent imaging of NF-κB activity and serum cytokine levels predict pain sensitivities in a rodent model of osteoarthritis.

dc.contributor.author Bowles, Robby D
dc.contributor.author Mata, Brian A
dc.contributor.author Bell, Richard D
dc.contributor.author Mwangi, Timothy K
dc.contributor.author Huebner, Janet L
dc.contributor.author Kraus, Virginia B
dc.contributor.author Setton, Lori A
dc.date.accessioned 2013-12-05T14:25:59Z
dc.date.issued 2013-11-18
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/24249543
dc.identifier.uri https://hdl.handle.net/10161/8165
dc.description.abstract Objective: To investigate the relationship between NF-κB activity, cytokine levels, and pain sensitivities in a rodent model of osteoarthritis (OA). Method: OA was induced in transgenic NF-κB luciferase reporter mice via mono-iodoacetate (MIA) intra-articular injection. Using luminescent imaging we evaluated the temporal kinetics of NF-κB activity and its relationship to the development of pain sensitivities and serum cytokine levels in this model. Results: MIA induced a transient increase in joint-related NF-кB activity at early time points (day 3 post-injection) and an associated biphasic pain (mechanical allodynia) response. NF-кB activity, serum IL-6, IL-1β, and IL-10 accounted for ~75% of the variability in pain-related mechanical sensitivities in this model. Specifically, NF-кB activity was strongly correlated to mechanical allodynia and serum IL-6 levels in the inflammatory pain phase of this model (day 3), while serum IL-1β was strongly correlated to pain sensitivities in the chronic pain phase of the model (day 28). Conclusion: Our findings suggest that NF-кB activity, IL-6 and IL-1β may be playing distinct roles in pain sensitivity development in this model of arthritis and may act to distinguish the acute from chronic pain phases of this model. This work establishes luminescent imaging of NF-кB activity as a novel imaging biomarker of pain sensitivities in this model of OA. © 2013 American College of Rheumatology.
dc.language ENG
dc.publisher Wiley
dc.relation.ispartof Arthritis Rheum
dc.relation.isversionof 10.1002/art.38279
dc.title In Vivo luminescent imaging of NF-κB activity and serum cytokine levels predict pain sensitivities in a rodent model of osteoarthritis.
dc.type Journal article
duke.contributor.id Kraus, Virginia B|0078823
duke.contributor.id Setton, Lori A|0117045
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/24249543
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Duke Molecular Physiology Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Rheumatology and Immunology
pubs.organisational-group Orthopaedics
pubs.organisational-group Pathology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
dc.identifier.eissn 1529-0131
duke.contributor.orcid Kraus, Virginia B|0000-0001-8173-8258


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