Skip to main content
Duke University Libraries
DukeSpace Scholarship by Duke Authors
  • Login
  • Ask
  • Menu
  • Login
  • Ask a Librarian
  • Search & Find
  • Using the Library
  • Research Support
  • Course Support
  • Libraries
  • About
View Item 
  •   DukeSpace
  • Duke Scholarly Works
  • Scholarly Articles
  • View Item
  •   DukeSpace
  • Duke Scholarly Works
  • Scholarly Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

An immunoglobulin C kappa-reactive single chain antibody fusion protein induces tolerance through receptor editing in a normal polyclonal immune system.

Thumbnail
View / Download
1.9 Mb
Date
2005-03-07
Authors
Ait-Azzouzene, Djemel
Verkoczy, Laurent
Peters, Jorieke
Gavin, Amanda
Skog, Patrick
Vela, José Luis
Nemazee, David
Repository Usage Stats
224
views
123
downloads
Abstract
Understanding immune tolerance mechanisms is a major goal of immunology research, but mechanistic studies have generally required the use of mouse models carrying untargeted or targeted antigen receptor transgenes, which distort lymphocyte development and therefore preclude analysis of a truly normal immune system. Here we demonstrate an advance in in vivo analysis of immune tolerance that overcomes these shortcomings. We show that custom superantigens generated by single chain antibody technology permit the study of tolerance in a normal, polyclonal immune system. In the present study we generated a membrane-tethered anti-Igkappa-reactive single chain antibody chimeric gene and expressed it as a transgene in mice. B cell tolerance was directly characterized in the transgenic mice and in radiation bone marrow chimeras in which ligand-bearing mice served as recipients of nontransgenic cells. We find that the ubiquitously expressed, Igkappa-reactive ligand induces efficient B cell tolerance primarily or exclusively by receptor editing. We also demonstrate the unique advantages of our model in the genetic and cellular analysis of immune tolerance.
Type
Journal article
Subject
Animals
Autoantigens
B-Lymphocytes
Bone Marrow Cells
Bone Marrow Transplantation
Cell Line, Tumor
Genes, RAG-1
Immune System
Immune Tolerance
Immunoglobulin kappa-Chains
Immunoglobulin lambda-Chains
Mice
Mice, Knockout
Mice, Transgenic
Mutation
Proto-Oncogene Proteins c-bcl-2
Rats
Recombinant Fusion Proteins
Permalink
https://hdl.handle.net/10161/8353
Published Version (Please cite this version)
10.1084/jem.20041854
Publication Info
Ait-Azzouzene, Djemel; Verkoczy, Laurent; Peters, Jorieke; Gavin, Amanda; Skog, Patrick; Vela, José Luis; & Nemazee, David (2005). An immunoglobulin C kappa-reactive single chain antibody fusion protein induces tolerance through receptor editing in a normal polyclonal immune system. J Exp Med, 201(5). pp. 817-828. 10.1084/jem.20041854. Retrieved from https://hdl.handle.net/10161/8353.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
Collections
  • Scholarly Articles
More Info
Show full item record

Scholars@Duke

Verkoczy

Laurent Karl Verkoczy

Adjunct Professor in the Department of Medicine
Laurent Verkoczy, PhD is Associate Professor of Medicine and Pathology at Duke University Medical Center. Dr.Verkoczy directs the Laboratory of B-cell Immunoregulation at the Duke Human Vaccine Institute and also serves as a B-cell Focus Investigator in Duke’s Center for HIV/AIDS Vaccine Immunology & Immunogen Discovery (CHAVI-ID) consortium.  He obtained his Ph.D. in Immunology from the University of Toronto in 2000 and completed post-doctoral studies at The S
This author no longer has a Scholars@Duke profile, so the information shown here reflects their Duke status at the time this item was deposited.
Open Access

Articles written by Duke faculty are made available through the campus open access policy. For more information see: Duke Open Access Policy

Rights for Collection: Scholarly Articles


Works are deposited here by their authors, and represent their research and opinions, not that of Duke University. Some materials and descriptions may include offensive content. More info

Make Your Work Available Here

How to Deposit

Browse

All of DukeSpaceCommunities & CollectionsAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit DateThis CollectionAuthorsTitlesTypesBy Issue DateDepartmentsAffiliations of Duke Author(s)SubjectsBy Submit Date

My Account

LoginRegister

Statistics

View Usage Statistics
Duke University Libraries

Contact Us

411 Chapel Drive
Durham, NC 27708
(919) 660-5870
Perkins Library Service Desk

Digital Repositories at Duke

  • Report a problem with the repositories
  • About digital repositories at Duke
  • Accessibility Policy
  • Deaccession and DMCA Takedown Policy

TwitterFacebookYouTubeFlickrInstagramBlogs

Sign Up for Our Newsletter
  • Re-use & Attribution / Privacy
  • Harmful Language Statement
  • Support the Libraries
Duke University