Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase.
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Degradation of specific protein substrates by the anaphase-promoting complex/cyclosome (APC) is critical for mitotic exit. We have identified the protein Xenopus nuclear factor 7 (Xnf7) as a novel APC inhibitor able to regulate the timing of exit from mitosis. Immunodepletion of Xnf7 from Xenopus laevis egg extracts accelerated the degradation of APC substrates cyclin B1, cyclin B2, and securin upon release from cytostatic factor arrest, whereas excess Xnf7 inhibited APC activity. Interestingly, Xnf7 exhibited intrinsic ubiquitin ligase activity, and this activity was required for APC inhibition. Unlike other reported APC inhibitors, Xnf7 did not associate with Cdc20, but rather bound directly to core subunits of the APC. Furthermore, Xnf7 was required for spindle assembly checkpoint function in egg extracts. These data suggest that Xnf7 is an APC inhibitor able to link spindle status to the APC through direct association with APC core components.
Ubiquitin-Protein Ligase Complexes
Published Version (Please cite this version)10.1083/jcb.200411056
Publication InfoCasaletto, JB; Nutt, LK; Wu, Q; Moore, JD; Etkin, LD; Jackson, PK; ... Kornbluth, S (2005). Inhibition of the anaphase-promoting complex by the Xnf7 ubiquitin ligase. J Cell Biol, 169(1). pp. 61-71. 10.1083/jcb.200411056. Retrieved from http://hdl.handle.net/10161/8379.
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Jo Rae Wright University Professor
Our lab studies the regulation of complex cellular processes, including cell cycle progression and programmed cell death (apoptosis). These tightly orchestrated processes are critical for appropriate cell proliferation and cell death, and when they go awry can result in cancer and degenerative disorders. Within these larger fields, we have focused on understanding the cellular mechanisms that prevent the onset of mitosis prior to the completion of DNA replication, the process
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