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    Mitochondrial fusion is regulated by Reaper to modulate Drosophila programmed cell death.

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    Date
    2011-10
    Authors
    Thomenius, M
    Freel, CD
    Horn, S
    Krieser, R
    Abdelwahid, E
    Cannon, R
    Balasundaram, S
    White, K
    Kornbluth, S
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    Abstract
    In most multicellular organisms, the decision to undergo programmed cell death in response to cellular damage or developmental cues is typically transmitted through mitochondria. It has been suggested that an exception is the apoptotic pathway of Drosophila melanogaster, in which the role of mitochondria remains unclear. Although IAP antagonists in Drosophila such as Reaper, Hid and Grim may induce cell death without mitochondrial membrane permeabilization, it is surprising that all three localize to mitochondria. Moreover, induction of Reaper and Hid appears to result in mitochondrial fragmentation during Drosophila cell death. Most importantly, disruption of mitochondrial fission can inhibit Reaper and Hid-induced cell death, suggesting that alterations in mitochondrial dynamics can modulate cell death in fly cells. We report here that Drosophila Reaper can induce mitochondrial fragmentation by binding to and inhibiting the pro-fusion protein MFN2 and its Drosophila counterpart dMFN/Marf. Our in vitro and in vivo analyses reveal that dMFN overexpression can inhibit cell death induced by Reaper or γ-irradiation. In addition, knockdown of dMFN causes a striking loss of adult wing tissue and significant apoptosis in the developing wing discs. Our findings are consistent with a growing body of work describing a role for mitochondrial fission and fusion machinery in the decision of cells to die.
    Type
    Journal article
    Subject
    Animals
    Apoptosis
    Cell Line
    Drosophila Proteins
    Drosophila melanogaster
    Gamma Rays
    HeLa Cells
    Humans
    Membrane Proteins
    Mitochondria
    Neuropeptides
    Protein Binding
    Permalink
    https://hdl.handle.net/10161/8381
    Published Version (Please cite this version)
    10.1038/cdd.2011.26
    Publication Info
    Thomenius, M; Freel, CD; Horn, S; Krieser, R; Abdelwahid, E; Cannon, R; ... Kornbluth, S (2011). Mitochondrial fusion is regulated by Reaper to modulate Drosophila programmed cell death. Cell Death Differ, 18(10). pp. 1640-1650. 10.1038/cdd.2011.26. Retrieved from https://hdl.handle.net/10161/8381.
    This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
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    Scholars@Duke

    Kornbluth

    Sally A. Kornbluth

    Jo Rae Wright University Professor
    Our lab studies the regulation of complex cellular processes, including cell cycle progression and programmed cell death (apoptosis). These tightly orchestrated processes are critical for appropriate cell proliferation and cell death, and when they go awry can result in cancer and degenerative disorders. Within these larger fields, we have focused on understanding the cellular mechanisms that prevent the onset of mitosis prior to the completion of DNA replication, the process
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