Biomimetic engineered muscle with capacity for vascular integration and functional maturation in vivo.
Abstract
Tissue-engineered skeletal muscle can serve as a physiological model of natural muscle
and a potential therapeutic vehicle for rapid repair of severe muscle loss and injury.
Here, we describe a platform for engineering and testing highly functional biomimetic
muscle tissues with a resident satellite cell niche and capacity for robust myogenesis
and self-regeneration in vitro. Using a mouse dorsal window implantation model and
transduction with fluorescent intracellular calcium indicator, GCaMP3, we nondestructively
monitored, in real time, vascular integration and the functional state of engineered
muscle in vivo. During a 2-wk period, implanted engineered muscle exhibited a steady
ingrowth of blood-perfused microvasculature along with an increase in amplitude of
calcium transients and force of contraction. We also demonstrated superior structural
organization, vascularization, and contractile function of fully differentiated vs.
undifferentiated engineered muscle implants. The described in vitro and in vivo models
of biomimetic engineered muscle represent enabling technology for novel studies of
skeletal muscle function and regeneration.
Type
Journal articleSubject
angiogenesiscontractile force
self-repair
tissue engineering
window chamber
Animals
Biomimetics
Cobra Cardiotoxin Proteins
Mice
Mice, Nude
Microvessels
Muscle Contraction
Muscle Development
Muscle, Skeletal
Tissue Engineering
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https://hdl.handle.net/10161/8413Published Version (Please cite this version)
10.1073/pnas.1402723111Publication Info
Juhas, Mark; Engelmayr, George C; Fontanella, Andrew N; Palmer, Gregory M; & Bursac,
Nenad (2014). Biomimetic engineered muscle with capacity for vascular integration and functional
maturation in vivo. Proc Natl Acad Sci U S A, 111(15). pp. 5508-5513. 10.1073/pnas.1402723111. Retrieved from https://hdl.handle.net/10161/8413.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Nenad Bursac
Professor of Biomedical Engineering
Bursac's research interests include: Stem cell, tissue engineering, and gene based
therapies for heart and muscle regeneration; Cardiac electrophysiology and arrhythmias;
Organ-on-chip and tissue engineering technologies for disease modeling and therapeutic
screening; Small and large animal models of heart and muscle injury, disease, and
regeneration.
The focus of my research is on application of pluripotent stem cells, tissue engineering,
and gene therapy technologies for: 1) basic s
Gregory M. Palmer
Associate Professor of Radiation Oncology
Greg Palmer obtained his B.S. in Biomedical Engineering from Marquette University
in 2000, after which he obtained his Ph.D. in BME from the University of Wisconsin,
Madison. He is currently an Associate Professor in the Department of Radiation Oncology,
Cancer Biology Division at Duke University Medical Center. His primary research focus
has been identifying and exploiting the changes in absorption, scattering, and fluorescence
properties of tissue associated with cancer progression and therape
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