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WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.

dc.contributor.author Jiang, Wei
dc.contributor.author Zhang, Donghui
dc.contributor.author Bursac, Nenad
dc.contributor.author Zhang, Yi
dc.coverage.spatial United States
dc.date.accessioned 2014-04-16T15:27:25Z
dc.date.issued 2013
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/24052941
dc.identifier S2213-6711(13)00004-0
dc.identifier.uri https://hdl.handle.net/10161/8425
dc.description.abstract Generation of functional cells from human pluripotent stem cells (PSCs) through in vitro differentiation is a promising approach for drug screening and cell therapy. However, the observed large and unavoidable variation in the differentiation potential of different human embryonic stem cell (hESC)/induced PSC (iPSC) lines makes the selection of an appropriate cell line for the differentiation of a particular cell lineage difficult. Here, we report identification of WNT3 as a biomarker capable of predicting definitive endoderm (DE) differentiation potential of hESCs. We show that the mRNA level of WNT3 in hESCs correlates with their DE differentiation efficiency. In addition, manipulations of hESCs through WNT3 knockdown or overexpression can respectively inhibit or promote DE differentiation in a WNT3 level-dependent manner. Finally, analysis of several hESC lines based on their WNT3 expression levels allowed accurate prediction of their DE differentiation potential. Collectively, our study supports the notion that WNT3 can serve as a biomarker for predicting DE differentiation potential of hESCs.
dc.language eng
dc.publisher Elsevier BV
dc.relation.ispartof Stem Cell Reports
dc.relation.isversionof 10.1016/j.stemcr.2013.03.003
dc.subject Biomarkers
dc.subject Cell Differentiation
dc.subject Cell Lineage
dc.subject Cells, Cultured
dc.subject Embryonic Stem Cells
dc.subject Endoderm
dc.subject Humans
dc.subject Induced Pluripotent Stem Cells
dc.subject RNA, Messenger
dc.subject Wnt3 Protein
dc.title WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.
dc.type Journal article
duke.contributor.id Bursac, Nenad|0312267
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/24052941
pubs.begin-page 46
pubs.end-page 52
pubs.issue 1
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.publication-status Published online
pubs.volume 1


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