Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts.
Repository Usage Stats
Stem cell transplantation holds great promise for the treatment of myocardial infarction injury. We recently described the embryonic stem cell-derived cardiac progenitor cells (CPCs) capable of differentiating into cardiomyocytes, vascular endothelium, and smooth muscle. In this study, we hypothesized that transplanted CPCs will preserve function of the infarcted heart by participating in both muscle replacement and neovascularization. Differentiated CPCs formed functional electromechanical junctions with cardiomyocytes in vitro and conducted action potentials over cm-scale distances. When transplanted into infarcted mouse hearts, CPCs engrafted long-term in the infarct zone and surrounding myocardium without causing teratomas or arrhythmias. The grafted cells differentiated into cross-striated cardiomyocytes forming gap junctions with the host cells, while also contributing to neovascularization. Serial echocardiography and pressure-volume catheterization demonstrated attenuated ventricular dilatation and preserved left ventricular fractional shortening, systolic and diastolic function. Our results demonstrate that CPCs can engraft, differentiate, and preserve the functional output of the infarcted heart.
Disease Models, Animal
Embryonic Stem Cells
Reverse Transcriptase Polymerase Chain Reaction
Stem Cell Transplantation
Published Version (Please cite this version)10.1371/journal.pone.0011536
Publication InfoChristoforou, Nicolas; Oskouei, Behzad N; Esteso, Paul; Hill, Christine M; Zimmet, Jeffrey M; Bian, Weining; ... Gearhart, John D (2010). Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts. PLoS One, 5(7). pp. e11536. 10.1371/journal.pone.0011536. Retrieved from https://hdl.handle.net/10161/8426.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
More InfoShow full item record
Professor of Biomedical Engineering
Bursac's research interests include: Stem cell, tissue engineering, and gene based therapies for heart and muscle regeneration; Cardiac electrophysiology and arrhythmias; Organ-on-chip and tissue engineering technologies for disease modeling and therapeutic screening; Small and large animal models of heart and muscle injury, disease, and regeneration. The focus of my research is on application of pluripotent stem cells, tissue engineering, and gene therapy technologies for: 1) basic s
Adjunct Professor of Biomedical Engineering
Professor Leong's research interest focuses on biomaterials design, particularly on synthesis of nanoparticles for DNA-based therapeutics, and nanostructured biomaterials for regenerative medicine Biomaterials Design: design of self-assembled fibers for tissue engineering microfluidics-mediated synthesis of multifunctional nanoparticles for drug and gene delivery synthesis of novel quantum dots for biomedical applications Con
This author no longer has a Scholars@Duke profile, so the information shown here reflects their Duke status at the time this item was deposited.
Alphabetical list of authors with Scholars@Duke profiles.