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Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts.

dc.contributor.author Bian, W
dc.contributor.author Bursac, Nenad
dc.contributor.author Christoforou, N
dc.contributor.author Esteso, P
dc.contributor.author Gearhart, JD
dc.contributor.author Hare, JM
dc.contributor.author Hill, CM
dc.contributor.author Leong, KW
dc.contributor.author Oskouei, BN
dc.contributor.author Zimmet, JM
dc.coverage.spatial United States
dc.date.accessioned 2011-06-21T17:31:32Z
dc.date.accessioned 2014-04-16T15:51:14Z
dc.date.issued 2010-07-12
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/20634944
dc.identifier.uri https://hdl.handle.net/10161/8426
dc.description.abstract Stem cell transplantation holds great promise for the treatment of myocardial infarction injury. We recently described the embryonic stem cell-derived cardiac progenitor cells (CPCs) capable of differentiating into cardiomyocytes, vascular endothelium, and smooth muscle. In this study, we hypothesized that transplanted CPCs will preserve function of the infarcted heart by participating in both muscle replacement and neovascularization. Differentiated CPCs formed functional electromechanical junctions with cardiomyocytes in vitro and conducted action potentials over cm-scale distances. When transplanted into infarcted mouse hearts, CPCs engrafted long-term in the infarct zone and surrounding myocardium without causing teratomas or arrhythmias. The grafted cells differentiated into cross-striated cardiomyocytes forming gap junctions with the host cells, while also contributing to neovascularization. Serial echocardiography and pressure-volume catheterization demonstrated attenuated ventricular dilatation and preserved left ventricular fractional shortening, systolic and diastolic function. Our results demonstrate that CPCs can engraft, differentiate, and preserve the functional output of the infarcted heart.
dc.language eng
dc.language.iso en_US
dc.relation.ispartof PLoS One
dc.relation.isversionof 10.1371/journal.pone.0011536
dc.relation.replaces http://hdl.handle.net/10161/4551
dc.relation.replaces 10161/4551
dc.subject Animals
dc.subject Cell Differentiation
dc.subject Cell Line
dc.subject Cells, Cultured
dc.subject Disease Models, Animal
dc.subject Echocardiography
dc.subject Embryonic Stem Cells
dc.subject Female
dc.subject Kaplan-Meier Estimate
dc.subject Mice
dc.subject Myocardial Infarction
dc.subject Myocytes, Cardiac
dc.subject Reverse Transcriptase Polymerase Chain Reaction
dc.subject Stem Cell Transplantation
dc.title Implantation of mouse embryonic stem cell-derived cardiac progenitor cells preserves function of infarcted murine hearts.
dc.title.alternative
dc.type Journal article
dc.description.version Version of Record
duke.date.pubdate 2010-7-12
duke.description.issue 7
duke.description.volume 5
dc.relation.journal Plos One
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/20634944
pubs.begin-page e11536
pubs.issue 7
pubs.organisational-group Biomedical Engineering
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Institutes and Centers
pubs.organisational-group Medicine
pubs.organisational-group Medicine, Cardiology
pubs.organisational-group Pratt School of Engineering
pubs.organisational-group School of Medicine
pubs.publication-status Published online
pubs.volume 5
dc.identifier.eissn 1932-6203


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