Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species.
Abstract
Cells are fundamental units of life, but little is known about evolution of cell states.
Induced pluripotent stem cells (iPSCs) are once differentiated cells that have been
re-programmed to an embryonic stem cell-like state, providing a powerful platform
for biology and medicine. However, they have been limited to a few mammalian species.
Here we found that a set of four mammalian transcription factor genes used to generate
iPSCs in mouse and humans can induce a partially reprogrammed pluripotent stem cell
(PRPSCs) state in vertebrate and invertebrate model organisms, in mammals, birds,
fish, and fly, which span 550 million years from a common ancestor. These findings
are one of the first to show cross-lineage stem cell-like induction, and to generate
pluripotent-like cells for several of these species with in vivo chimeras. We suggest
that the stem-cell state may be highly conserved across a wide phylogenetic range.
DOI:http://dx.doi.org/10.7554/eLife.00036.001.
Type
Journal articleSubject
ChickenDrosophila
Mouse
Zebrafish
iPS
quail
zebra finch
Animals
Biomarkers
Cell Lineage
Cell Proliferation
Cells, Cultured
Cellular Reprogramming
Chick Embryo
Chimera
Drosophila
Embryonic Stem Cells
Finches
Gene Expression Regulation, Developmental
Genotype
Induced Pluripotent Stem Cells
Karyotyping
Kruppel-Like Transcription Factors
Mice
Octamer Transcription Factor-3
Phenotype
Proto-Oncogene Proteins c-myc
Quail
SOXB1 Transcription Factors
Telomerase
Transcription Factors
Transfection
Zebrafish
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https://hdl.handle.net/10161/9312Published Version (Please cite this version)
10.7554/eLife.00036Publication Info
Rosselló, Ricardo Antonio; Chen, Chun-Chun; Dai, Rui; Howard, Jason T; Hochgeschwender,
Ute; & Jarvis, Erich D (2013). Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian
vertebrate and invertebrate species. Elife, 2. pp. e00036. 10.7554/eLife.00036. Retrieved from https://hdl.handle.net/10161/9312.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Erich David Jarvis
Adjunct Professor in the Dept. of Neurobiology
Dr. Jarvis' laboratory studies the neurobiology of vocal communication. Emphasis is
placed on the molecular pathways involved in the perception and production of learned
vocalizations. They use an integrative approach that combines behavioral, anatomical,
electrophysiological and molecular biological techniques. The main animal model used
is songbirds, one of the few vertebrate groups that evolved the ability to learn vocalizations.
The generality of the discoveries is tested in other vocal

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