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Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species.

dc.contributor.author Chen, CC
dc.contributor.author Dai, R
dc.contributor.author Hochgeschwender, U
dc.contributor.author Howard, JT
dc.contributor.author Jarvis, Erich David
dc.contributor.author Rosselló, RA
dc.coverage.spatial England
dc.date.accessioned 2014-12-15T16:52:23Z
dc.date.issued 2013-09-03
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/24015354
dc.identifier 00036
dc.identifier.uri http://hdl.handle.net/10161/9312
dc.description.abstract Cells are fundamental units of life, but little is known about evolution of cell states. Induced pluripotent stem cells (iPSCs) are once differentiated cells that have been re-programmed to an embryonic stem cell-like state, providing a powerful platform for biology and medicine. However, they have been limited to a few mammalian species. Here we found that a set of four mammalian transcription factor genes used to generate iPSCs in mouse and humans can induce a partially reprogrammed pluripotent stem cell (PRPSCs) state in vertebrate and invertebrate model organisms, in mammals, birds, fish, and fly, which span 550 million years from a common ancestor. These findings are one of the first to show cross-lineage stem cell-like induction, and to generate pluripotent-like cells for several of these species with in vivo chimeras. We suggest that the stem-cell state may be highly conserved across a wide phylogenetic range. DOI:http://dx.doi.org/10.7554/eLife.00036.001.
dc.language eng
dc.relation.ispartof Elife
dc.relation.isversionof 10.7554/eLife.00036
dc.subject Chicken
dc.subject Drosophila
dc.subject Mouse
dc.subject Zebrafish
dc.subject iPS
dc.subject quail
dc.subject zebra finch
dc.subject Animals
dc.subject Biomarkers
dc.subject Cell Lineage
dc.subject Cell Proliferation
dc.subject Cells, Cultured
dc.subject Cellular Reprogramming
dc.subject Chick Embryo
dc.subject Chimera
dc.subject Drosophila
dc.subject Embryonic Stem Cells
dc.subject Finches
dc.subject Gene Expression Regulation, Developmental
dc.subject Genotype
dc.subject Induced Pluripotent Stem Cells
dc.subject Karyotyping
dc.subject Kruppel-Like Transcription Factors
dc.subject Mice
dc.subject Octamer Transcription Factor-3
dc.subject Phenotype
dc.subject Proto-Oncogene Proteins c-myc
dc.subject Quail
dc.subject SOXB1 Transcription Factors
dc.subject Telomerase
dc.subject Transcription Factors
dc.subject Transfection
dc.subject Zebrafish
dc.title Mammalian genes induce partially reprogrammed pluripotent stem cells in non-mammalian vertebrate and invertebrate species.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/24015354
pubs.begin-page e00036
pubs.organisational-group Basic Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Faculty
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Neurobiology
pubs.organisational-group School of Medicine
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published online
pubs.volume 2


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