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Astrocytes refine cortical connectivity at dendritic spines.

dc.contributor.author Bhagat, S
dc.contributor.author Calakos, Nicole
dc.contributor.author Calhan, OY
dc.contributor.author Eroglu, C
dc.contributor.author Kim, IH
dc.contributor.author Patel, S
dc.contributor.author Pilaz, LJ
dc.contributor.author Risher, WC
dc.contributor.author Silver, Debra Lynn
dc.contributor.author Singh Alvarado, J
dc.contributor.author Soderling, SH
dc.contributor.author Stevens, B
dc.contributor.author Uezu, A
dc.contributor.author Wilton, DK
dc.coverage.spatial England
dc.date.accessioned 2015-01-15T19:23:13Z
dc.date.issued 2014-12-17
dc.identifier http://www.ncbi.nlm.nih.gov/pubmed/25517933
dc.identifier.uri https://hdl.handle.net/10161/9362
dc.description.abstract During cortical synaptic development, thalamic axons must establish synaptic connections despite the presence of the more abundant intracortical projections. How thalamocortical synapses are formed and maintained in this competitive environment is unknown. Here, we show that astrocyte-secreted protein hevin is required for normal thalamocortical synaptic connectivity in the mouse cortex. Absence of hevin results in a profound, long-lasting reduction in thalamocortical synapses accompanied by a transient increase in intracortical excitatory connections. Three-dimensional reconstructions of cortical neurons from serial section electron microscopy (ssEM) revealed that, during early postnatal development, dendritic spines often receive multiple excitatory inputs. Immuno-EM and confocal analyses revealed that majority of the spines with multiple excitatory contacts (SMECs) receive simultaneous thalamic and cortical inputs. Proportion of SMECs diminishes as the brain develops, but SMECs remain abundant in Hevin-null mice. These findings reveal that, through secretion of hevin, astrocytes control an important developmental synaptic refinement process at dendritic spines.
dc.language eng
dc.relation.ispartof Elife
dc.relation.isversionof 10.7554/eLife.04047
dc.subject astrocytes
dc.subject cell biology
dc.subject dendritic spines
dc.subject mouse
dc.subject neuroscience
dc.subject synaptogenesis
dc.subject thalamocortical
dc.subject Animals
dc.subject Astrocytes
dc.subject Calcium-Binding Proteins
dc.subject Dendritic Spines
dc.subject Extracellular Matrix Proteins
dc.subject Mice
dc.subject Microscopy, Confocal
dc.subject Microscopy, Immunoelectron
dc.subject Synapses
dc.title Astrocytes refine cortical connectivity at dendritic spines.
dc.type Journal article
pubs.author-url http://www.ncbi.nlm.nih.gov/pubmed/25517933
pubs.organisational-group Basic Science Departments
pubs.organisational-group Cell Biology
pubs.organisational-group Clinical Science Departments
pubs.organisational-group Duke
pubs.organisational-group Duke Cancer Institute
pubs.organisational-group Duke Institute for Brain Sciences
pubs.organisational-group Institutes and Centers
pubs.organisational-group Institutes and Provost's Academic Units
pubs.organisational-group Molecular Genetics and Microbiology
pubs.organisational-group Neurobiology
pubs.organisational-group Neurology
pubs.organisational-group Neurology, Movement Disorders
pubs.organisational-group School of Medicine
pubs.organisational-group Staff
pubs.organisational-group University Institutes and Centers
pubs.publication-status Published online
pubs.volume 3
dc.identifier.eissn 2050-084X


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