Characterization of basal pseudopod-like processes in ileal and colonic PYY cells.
Abstract
The peptide tyrosine tyrosine (PYY) is produced and secreted from L cells of the gastrointestinal
mucosa. To study the anatomy and function of PYY-secreting L cells, we developed a
transgenic PYY-green fluorescent protein mouse model. PYY-containing cells exhibited
green fluorescence under UV light and were immunoreactive to antibodies against PYY
and GLP-1 (glucagon-like peptide-1, an incretin hormone also secreted by L cells).
PYY-GFP cells from 15 μm thick sections were imaged using confocal laser scanning
microscopy and three-dimensionally (3D) reconstructed. Results revealed unique details
of the anatomical differences between ileal and colonic PYY-GFP cells. In ileal villi,
the apical portion of PYY cells makes minimal contact with the lumen of the gut. Long
pseudopod-like basal processes extend from these cells and form an interface between
the mucosal epithelium and the lamina propria. Some basal processes are up to 50 μm
in length. Multiple processes can be seen protruding from one cell and these often
have a terminus resembling a synapse that appears to interact with neighboring cells.
In colonic crypts, PYY-GFP cells adopt a spindle-like shape and weave in between epithelial
cells, while maintaining contact with the lumen and lamina propria. In both tissues,
cytoplasmic granules containing the hormones PYY and GLP-1 are confined to the base
of the cell, often filling the basal process. The anatomical arrangement of these
structures suggests a dual function as a dock for receptors to survey absorbed nutrients
and as a launching platform for hormone secretion in a paracrine fashion.
Type
Journal articleSubject
AnimalsColon
Enteroendocrine Cells
Glucagon-Like Peptide 1
Green Fluorescent Proteins
Ileum
Intestinal Mucosa
Mice
Mice, Transgenic
Peptide YY
Protein Transport
Pseudopodia
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https://hdl.handle.net/10161/9383Published Version (Please cite this version)
10.1007/s10735-010-9302-6Publication Info
Bohórquez, Diego V; Chandra, Rashmi; Samsa, Leigh Ann; Vigna, Steven R; & Liddle,
Rodger A (2011). Characterization of basal pseudopod-like processes in ileal and colonic PYY cells.
J Mol Histol, 42(1). pp. 3-13. 10.1007/s10735-010-9302-6. Retrieved from https://hdl.handle.net/10161/9383.This is constructed from limited available data and may be imprecise. To cite this
article, please review & use the official citation provided by the journal.
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Show full item recordScholars@Duke
Diego V. Bohorquez
Associate Professor in Medicine
I am a gut-brain neuroscientist. Though my initial studies focused on GI physiology
and nutrition, my expertise evolved to include neuroscience following the many personal
stories, which have carefully sharpened my career vision along the way. While pursuing
a Doctoral degree in Nutrition, a friend shared her struggles with obesity and gastric
bypass surgery. Surgery was a last resort but helped to reduced her body weight dramatically
and resolved her diabe
Rodger Alan Liddle
Professor of Medicine
Our laboratory has two major research interests:Enteroendocrine Cell Biology
Enteroendocrine cells (EECs) are sensory cells of the gut that send signals throughout
the body. They have the ability to sense food and nutrients in the lumen of the intestine
and secrete hormones into the blood. Our laboratory has had a longstanding interest
in two types of EECs that regulate satiety and signal the brain to stop eating. Chole
Steven R. Vigna
Associate Professor of Cell Biology
We are interested in the mechanisms of signal transduction of neuropeptides and
peptide hormones, mechanisms of receptor regulation, and the role of receptor regulation
in health and disease. The major peptide of interest is the neurotransmitter substance
P which is involved in gastrointestinal tract regulation, pain pathways, inflammation,
and central nervous system functions. Our major focus is at the level of the receptors
for this peptide. We are currently studying mechanisms of subst
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