Irisin - a myth rather than an exercise-inducible myokine.
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The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non-specific proteins in human and animal sera. Using recombinant glycosylated and non-glycosylated irisin as positive controls, we found no immune-reactive bands of the expected size in any biological samples. A FNDC5 signature was identified at ~20 kDa by mass spectrometry in human serum but was not detected by the commercial pAbs tested. Our results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species.
Blood Chemical Analysis
Enzyme-Linked Immunosorbent Assay
Reproducibility of Results
Sensitivity and Specificity
Published Version (Please cite this version)10.1038/srep08889
Publication InfoAlbrecht, E; Brenmoehl, J; Drevon, CA; Erickson, Harold Paul; Holen, T; Lee, S; ... Thomas, S (2015). Irisin - a myth rather than an exercise-inducible myokine. Sci Rep, 5. pp. 8889. 10.1038/srep08889. Retrieved from https://hdl.handle.net/10161/9501.
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James B. Duke Professor of Cell Biology
Cytoskeleton: It is now clear that the actin and microtubule cytoskeleton originated in bacteria. Our major research is on FtsZ, the bacterial tubulin homolog, which assembles into a contractile ring that divides the bacterium. We have studied FtsZ assembly in vitro, and found that it assembles into thin protofilaments (pfs). Dozens of these pfs are further clustered to form the contractile Z-ring in vivo. Some important discoveries in the last ten years include: &bul
Assistant Research Professor of Cell Biology
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