The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.

dc.contributor.author

Ni, Ting

dc.contributor.author

Tu, Kang

dc.contributor.author

Wang, Zhong

dc.contributor.author

Song, Shen

dc.contributor.author

Wu, Han

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Xie, Bin

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Scott, Kristin C

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Grewal, Shiv I

dc.contributor.author

Gao, Yuan

dc.contributor.author

Zhu, Jun

dc.coverage.spatial

United States

dc.date.accessioned

2011-06-21T17:32:20Z

dc.date.issued

2010-12-20

dc.description.abstract

A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe.

dc.description.version

Version of Record

dc.identifier

http://www.ncbi.nlm.nih.gov/pubmed/21187966

dc.identifier.eissn

1932-6203

dc.identifier.uri

https://hdl.handle.net/10161/4587

dc.language

eng

dc.language.iso

en_US

dc.publisher

Public Library of Science (PLoS)

dc.relation.ispartof

PLoS One

dc.relation.isversionof

10.1371/journal.pone.0015271

dc.relation.journal

Plos One

dc.subject

DNA Polymerase II

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Gene Expression Profiling

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Gene Expression Regulation, Fungal

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Gene Library

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Genes, Dominant

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Genes, Fungal

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Genome, Fungal

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Histones

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Meiosis

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Models, Genetic

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Oligonucleotides, Antisense

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RNA

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Reverse Transcriptase Polymerase Chain Reaction

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Saccharomyces cerevisiae Proteins

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Schizosaccharomyces

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Sequence Analysis, DNA

dc.title

The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.

dc.title.alternative
dc.type

Journal article

duke.date.pubdate

2010-12-20

duke.description.issue

12

duke.description.volume

5

pubs.author-url

http://www.ncbi.nlm.nih.gov/pubmed/21187966

pubs.begin-page

e15271

pubs.issue

12

pubs.organisational-group

Basic Science Departments

pubs.organisational-group

Duke

pubs.organisational-group

Molecular Genetics and Microbiology

pubs.organisational-group

School of Medicine

pubs.publication-status

Published online

pubs.volume

5

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