Proteomic analysis defines the interactome of telomerase in the protozoan parasite, <i>Trypanosoma brucei</i>.

dc.contributor.author

Davis, Justin A

dc.contributor.author

Reyes, Andres V

dc.contributor.author

Nitika

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Saha, Arpita

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Wolfgeher, Donald J

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Xu, Shou-Ling

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Truman, Andrew W

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Li, Bibo

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Chakrabarti, Kausik

dc.date.accessioned

2024-08-20T19:19:10Z

dc.date.available

2024-08-20T19:19:10Z

dc.date.issued

2023-01

dc.description.abstract

Telomerase is a ribonucleoprotein enzyme responsible for maintaining the telomeric end of the chromosome. The telomerase enzyme requires two main components to function: the telomerase reverse transcriptase (TERT) and the telomerase RNA (TR), which provides the template for telomeric DNA synthesis. TR is a long non-coding RNA, which forms the basis of a large structural scaffold upon which many accessory proteins can bind and form the complete telomerase holoenzyme. These accessory protein interactions are required for telomerase activity and regulation inside cells. The interacting partners of TERT have been well studied in yeast, human, and Tetrahymena models, but not in parasitic protozoa, including clinically relevant human parasites. Here, using the protozoan parasite, Trypanosoma brucei (T. brucei) as a model, we have identified the interactome of T. brucei TERT (TbTERT) using a mass spectrometry-based approach. We identified previously known and unknown interacting factors of TbTERT, highlighting unique features of T. brucei telomerase biology. These unique interactions with TbTERT, suggest mechanistic differences in telomere maintenance between T. brucei and other eukaryotes.

dc.identifier

1110423

dc.identifier.issn

2296-634X

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2296-634X

dc.identifier.uri

https://hdl.handle.net/10161/31420

dc.language

eng

dc.publisher

Frontiers Media SA

dc.relation.ispartof

Frontiers in cell and developmental biology

dc.relation.isversionof

10.3389/fcell.2023.1110423

dc.rights.uri

https://creativecommons.org/licenses/by-nc/4.0

dc.subject

Trypanosoma brucei

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cell growth

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cell proliferation

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interactome

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parasite

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ribonucleoprotein (RNP)

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telomerase

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telomere

dc.title

Proteomic analysis defines the interactome of telomerase in the protozoan parasite, Trypanosoma brucei.

dc.type

Journal article

pubs.begin-page

1110423

pubs.organisational-group

Duke

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Trinity College of Arts & Sciences

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Staff

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Chemistry

pubs.publication-status

Published

pubs.volume

11

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