A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium

dc.contributor.author

Roye, Yasmin

dc.contributor.author

Bhattacharya, Rohan

dc.contributor.author

Mou, Xingrui

dc.contributor.author

Zhou, Yuhao

dc.contributor.author

Burt, Morgan A

dc.contributor.author

Musah, Samira

dc.date.accessioned

2021-08-24T13:34:40Z

dc.date.available

2021-08-24T13:34:40Z

dc.date.updated

2021-08-24T13:34:37Z

dc.description.abstract

<jats:p>Progress in understanding kidney disease mechanisms and the development of targeted therapeutics have been limited by the lack of functional in vitro models that can closely recapitulate human physiological responses. Organ Chip (or organ-on-a-chip) microfluidic devices provide unique opportunities to overcome some of these challenges given their ability to model the structure and function of tissues and organs in vitro. Previously established organ chip models typically consist of heterogenous cell populations sourced from multiple donors, limiting their applications in patient-specific disease modeling and personalized medicine. In this study, we engineered a personalized glomerulus chip system reconstituted from human induced pluripotent stem (iPS) cell-derived vascular endothelial cells (ECs) and podocytes from a single patient. Our stem cell-derived kidney glomerulus chip successfully mimics the structure and some essential functions of the glomerular filtration barrier. We further modeled glomerular injury in our tissue chips by administering a clinically relevant dose of the chemotherapy drug Adriamycin. The drug disrupts the structural integrity of the endothelium and the podocyte tissue layers, leading to significant albuminuria as observed in patients with glomerulopathies. We anticipate that the personalized glomerulus chip model established in this report could help advance future studies of kidney disease mechanisms and the discovery of personalized therapies. Given the remarkable ability of human iPS cells to differentiate into almost any cell type, this work also provides a blueprint for the establishment of more personalized organ chip and ‘body-on-a-chip’ models in the future.</jats:p>

dc.identifier.issn

2072-666X

dc.identifier.uri

https://hdl.handle.net/10161/23648

dc.language

en

dc.publisher

MDPI AG

dc.relation.ispartof

Micromachines

dc.relation.isversionof

10.3390/mi12080967

dc.title

A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium

dc.type

Journal article

duke.contributor.orcid

Bhattacharya, Rohan|0000-0002-3663-9311

pubs.begin-page

967

pubs.end-page

967

pubs.issue

8

pubs.organisational-group

Student

pubs.organisational-group

Biomedical Engineering

pubs.organisational-group

Duke

pubs.organisational-group

Pratt School of Engineering

pubs.publication-status

Published online

pubs.volume

12

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
micromachines-12-00967.pdf
Size:
3.77 MB
Format:
Adobe Portable Document Format