CONSTAX2: Improved taxonomic classification of environmental DNA markers.

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2021-05-07

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Abstract

Summary

CONSTAX - the CONSensus TAXonomy classifier - was developed for accurate and reproducible taxonomic annotation of fungal rDNA amplicon sequences and is based upon a consensus approach of RDP, SINTAX, and UTAX algorithms. CONSTAX2 extends these features to classify prokaryotes as well as eukaryotes and incorporates BLAST-based classifiers to reduce classification errors. Additionally, CONSTAX2 implements a conda-installable command line tool with improved classification metrics, faster training, multithreading support, capacity to incorporate external taxonomic databases, and new isolate matching and high-level taxonomy tools, replete with documentation and example tutorials.

Availability

CONSTAX2 is available at https://github.com/liberjul/CONSTAXv2, and is packaged for Linux and MacOS from Bioconda with use under the MIT License. A tutorial and documentation are available at https://constax.readthedocs.io/en/latest/.

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Supplementary data are available at Bioinformatics online.

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Published Version (Please cite this version)

10.1093/bioinformatics/btab347

Publication Info

Liber, Julian A, Gregory Bonito and Gian Maria Niccolò Benucci (2021). CONSTAX2: Improved taxonomic classification of environmental DNA markers. Bioinformatics (Oxford, England), 37(21). pp. 3941–3943. 10.1093/bioinformatics/btab347 Retrieved from https://hdl.handle.net/10161/24215.

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