Dual impact of elevated temperature on plant defence and bacterial virulence in Arabidopsis.
Repository Usage Stats
Environmental conditions profoundly affect plant disease development; however, the underlying molecular bases are not well understood. Here we show that elevated temperature significantly increases the susceptibility of Arabidopsis to Pseudomonas syringae pv. tomato (Pst) DC3000 independently of the phyB/PIF thermosensing pathway. Instead, elevated temperature promotes translocation of bacterial effector proteins into plant cells and causes a loss of ICS1-mediated salicylic acid (SA) biosynthesis. Global transcriptome analysis reveals a major temperature-sensitive node of SA signalling, impacting ~60% of benzothiadiazole (BTH)-regulated genes, including ICS1 and the canonical SA marker gene, PR1. Remarkably, BTH can effectively protect Arabidopsis against Pst DC3000 infection at elevated temperature despite the lack of ICS1 and PR1 expression. Our results highlight the broad impact of a major climate condition on the enigmatic molecular interplay between temperature, SA defence and function of a central bacterial virulence system in the context of a widely studied susceptible plant-pathogen interaction.
Published Version (Please cite this version)
Huot, Bethany, Christian Danve M Castroverde, André C Velásquez, Emily Hubbard, Jane A Pulman, Jian Yao, Kevin L Childs, Kenichi Tsuda, et al. (2017). Dual impact of elevated temperature on plant defence and bacterial virulence in Arabidopsis. Nature communications, 8(1). p. 1808. 10.1038/s41467-017-01674-2 Retrieved from https://hdl.handle.net/10161/21719.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
Interested in the fascinating world of plants, microbes or inter-organismal communication and co-evolution? Please contact Prof. Sheng-Yang He (email@example.com; firstname.lastname@example.org).
Millions of years of co-evolution between plants and microbes have resulted in an intricate web of attack, counter-attack, decoy, and hijacking mechanisms in biology. Moreover, co-evolution between plants and microbes is greatly impacted by ongoing climate change. In our lab, we probe “host-microbe-climate” interactions to answer the following fundamental questions: (1) How do microbial pathogens infect a susceptible host? (2) How do plants select beneficial microbiomes to ensure health? (3) How do climate conditions impact disease and immunity?
We use contemporary methods to address these questions, including those commonly used in molecular genetics, genomics, biochemistry, cell biology, bioinformatics, microbiology, plant biology, co-evolution and infectious disease biology.
Unless otherwise indicated, scholarly articles published by Duke faculty members are made available here with a CC-BY-NC (Creative Commons Attribution Non-Commercial) license, as enabled by the Duke Open Access Policy. If you wish to use the materials in ways not already permitted under CC-BY-NC, please consult the copyright owner. Other materials are made available here through the author’s grant of a non-exclusive license to make their work openly accessible.