Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primates.
Date
2010-06-16
Editors
Journal Title
Journal ISSN
Volume Title
Repository Usage Stats
views
downloads
Citation Stats
Abstract
Medications that can mitigate against radiation injury are limited. In this study, we investigated the ability of recombinant human growth hormone (rhGH) to mitigate against radiation injury in mice and nonhuman primates. BALB/c mice were irradiated with 7.5 Gy and treated post-irradiation with rhGH intravenously at a once daily dose of 20 microg/dose for 35 days. rhGH protected 17 out of 28 mice (60.7%) from lethal irradiation while only 3 out of 28 mice (10.7%) survived in the saline control group. A shorter course of 5 days of rhGH post-irradiation produced similar results. Compared with the saline control group, treatment with rhGH on irradiated BALB/c mice significantly accelerated overall hematopoietic recovery. Specifically, the recovery of total white cells, CD4 and CD8 T cell subsets, B cells, NK cells and especially platelets post radiation exposure were significantly accelerated in the rhGH-treated mice. Moreover, treatment with rhGH increased the frequency of hematopoietic stem/progenitor cells as measured by flow cytometry and colony forming unit assays in bone marrow harvested at day 14 after irradiation, suggesting the effects of rhGH are at the hematopoietic stem/progenitor level. rhGH mediated the hematopoietic effects primarily through their niches. Similar data with rhGH were also observed following 2 Gy sublethal irradiation of nonhuman primates. Our data demonstrate that rhGH promotes hematopoietic engraftment and immune recovery post the exposure of ionizing radiation and mitigates against the mortality from lethal irradiation even when administered after exposure.
Type
Department
Description
Provenance
Citation
Permalink
Published Version (Please cite this version)
Publication Info
Chen, Benny J, Divino Deoliveira, Ivan Spasojevic, Gregory D Sempowski, Chen Jiang, Kouros Owzar, Xiaojuan Wang, Diane Gesty-Palmer, et al. (2010). Growth hormone mitigates against lethal irradiation and enhances hematologic and immune recovery in mice and nonhuman primates. PLoS One, 5(6). p. e11056. 10.1371/journal.pone.0011056 Retrieved from https://hdl.handle.net/10161/4547.
This is constructed from limited available data and may be imprecise. To cite this article, please review & use the official citation provided by the journal.
Collections
Scholars@Duke

Jun Chen

Ivan Spasojevic

Diane Gesty-Palmer
Dr. Gesty-Palmer is an Assistant Professor in the Department of Medicine and Division of Endocrinology and Metabolism. Her laboratory principally examines G protein-coupled receptor signaling mechanisms in bone metabolism with emphasis on parathyroid hormone (PTH) receptor biased agonism. These studies explore novel mechanisms of PTH receptor signaling and the contributions of β-arrestin to modulate bone formation and bone resorption. Her work has recently shown that β-arrestin, classically thought to turn off G protein signaling, activates a distinct G protein-independent signaling mechanism to stimulate bone formation. Examination of β-arrestin mediated signaling events may provide novel therapeutic targets for the treatment of metabolic bone diseases such as osteoporosis. Dr. Gesty-Palmer is the recipient of a BIRCWH Scholar Award for Interdisciplinary Research in Women’s Health in 2006 and an Arthritis Foundation Investigator Award in 2007.
Unless otherwise indicated, scholarly articles published by Duke faculty members are made available here with a CC-BY-NC (Creative Commons Attribution Non-Commercial) license, as enabled by the Duke Open Access Policy. If you wish to use the materials in ways not already permitted under CC-BY-NC, please consult the copyright owner. Other materials are made available here through the author’s grant of a non-exclusive license to make their work openly accessible.