Characterization complex collagen fiber architecture in knee joint using high-resolution diffusion imaging.

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2020-01-21

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Abstract

PURPOSE:To evaluate the complex fiber orientations and 3D collagen fiber network of knee joint connective tissues, including ligaments, muscle, articular cartilage, and meniscus using high spatial and angular resolution diffusion imaging. METHODS:Two rat knee joints were scanned using a modified 3D diffusion-weighted spin echo pulse sequence with the isotropic spatial resolution of 45 μm at 9.4T. The b values varied from 250 to 1250 s/mm2 with 31 diffusion encoding directions for 1 rat knee. The b value was fixed to 1000 s/mm2 with 147 diffusion encoding directions for the second knee. Both the diffusion tensor imaging (DTI) model and generalized Q-sampling imaging (GQI) method were used to investigate the fiber orientation distributions and tractography with the validation of polarized light microscopy. RESULTS:To better resolve the crossing fibers, the b value should be great than or equal to 1000 s/mm2 . The tractography results were comparable between the DTI model and GQI method in ligament and muscle. However, the tractography exhibited apparent difference between DTI and GQI in connective tissues with more complex collagen fibers network, such as cartilage and meniscus. In articular cartilage, there were numerous crossing fibers found in superficial zone and transitional zone. Tractography generated with GQI also resulted in more intact tracts in articular cartilage than DTI. CONCLUSION:High-resolution diffusion imaging with GQI method can trace the complex collagen fiber orientations and architectures of the knee joint at microscopic resolution.

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10.1002/mrm.28181

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Wang, Nian, Anthony J Mirando, Gary Cofer, Yi Qi, Matthew J Hilton and G Allan Johnson (2020). Characterization complex collagen fiber architecture in knee joint using high-resolution diffusion imaging. Magnetic resonance in medicine. 10.1002/mrm.28181 Retrieved from https://hdl.handle.net/10161/19899.

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Scholars@Duke

Hilton

Matthew James Hilton

Professor in Orthopaedic Surgery

A long-term interest of the Hilton lab is to uncover the molecular circuitry regulating lineage commitment, proliferation, and differentiation of skeletal stem cells, chondrocytes, and osteoblasts. My laboratory uses genetic mouse models and primary cell culture techniques coupled with biochemistry to answer questions regarding skeletal stem cell self-renewal/differentiation, chondrogenesis, and osteoblastogenesis. Recently my lab has generated novel data from a variety of Notch gain and loss-of-function mutant mice demonstrating the importance of Notch signaling in each of these processes. We are currently investigating the exact Notch signaling mechanisms at play during skeletal development, disease, and repair. Additional studies are also focused on identifying and understanding the molecular mechanisms underlying various congenital skeletal pathologies, including Multiple Herediatry Exostoses (MHE) and Preaxial Polydactyly (PPD).

Johnson

G. Allan Johnson

Charles E. Putman University Distinguished Professor of Radiology

Dr. Johnson is the Charles E. Putman University Professor of Radiology, Professor of Physics, and Biomedical Engineering, and Director of the Duke Center for In Vivo Microscopy (CIVM). The CIVM is an NIH/NIBIB national Biomedical Technology Resource Center with a mission to develop novel technologies for preclinical imaging (basic sciences) and apply the technologies to critical biomedical questions. Dr. Johnson was one of the first researchers to bring Paul Lauterbur's vision of magnetic resonance (MR) microscopy to practice as described in his paper, "Nuclear magnetic resonance imaging at microscopic resolution" (J Magn Reson 68:129-137, 1986). Dr. Johnson is involved in both the engineering physics required to extend the resolution of MR imaging and in a broad range of applications in the basic sciences.


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