SERS Detection of SARS‐CoV‐2 Spike Proteins and IgG Antibodies Using Metallic <i>H</i> ‐type Nano‐Apertures
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<jats:title>Abstract</jats:title> <jats:p> The rapid and accurate detection of viral biomarkers is critical for controlling infectious disease outbreaks, as highlighted by the COVID‐19 pandemic. Here, we present a label‐free platform for the ultrasensitive detection of SARS‐CoV‐2 spike protein and IgG antibodies in liquid using surface‐enhanced Raman spectroscopy (SERS) based on an array of plasmonic <jats:italic>H</jats:italic> ‐shaped nano‐apertures. The nanostructure is designed to support a resonance at 777.8 nm. The Raman signal of both biomolecules was recorded at several locations on the nanostructure under off‐resonance laser excitation at 785 nm, confirming its reproducibility. The designed nanostructure enables limit of detection of 0.85 ng/mL in a liquid environment, without the need for complex surface functionalization or labeling. Using principal component analysis (PCA), we successfully distinguished between spike protein, IgG antibodies, and their mixtures, highlighting the platform's ability to analyze complex biological matrices. This work advances the development of sensitive SERS‐based biosensors for the rapid and accurate detection of emerging pathogens in liquid environments. </jats:p>
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Gao, Yifei Effie, Zilin Jiang, Nikolaos K Ioannou and Domna G Kotsifaki (n.d.). SERS Detection of SARS‐CoV‐2 Spike Proteins and IgG Antibodies Using Metallic H ‐type Nano‐Apertures. Advanced Physics Research. 10.1002/apxr.202500143 Retrieved from https://hdl.handle.net/10161/33545.
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Scholars@Duke
Domna Kotsifaki
Her research focuses on optical manipulation and sensing at the micro- and nanoscale. At DKU, she leads a research group pioneering the use of plasmonic nanostructures and metamaterials for advanced biosensing—such as detecting viruses and proteins—and for environmental monitoring, including the identification of micro-/nano-plastics in local water systems. Her teaching interests at DKU include Biophysics, Optics and Modern Physics, as well as General Physics.
She has an extensive publication record in high-impact journals including Nano Letters, Nanophotonics, and Science of the Total Environment. Her research output since joining DKU in 2022 includes numerous articles in journals such as Advanced Physics Research, Heliyon, and Biomedical Optics Express, a significant proportion of which are co-authored by DKU undergraduate students. Her work is presented on the international stage through invited talks at major conferences, complemented by numerous contributed presentations. The impact of her mentorship is further demonstrated by her students receiving international poster awards and scholarships for their research. She is a recipient of a Marie Skłodowska-Curie Actions Seal of Excellence and has a strong track record of securing competitive research funding as PI, with awards from the Kunshan Government and the JSPS Kakenhi program. An active leader in her field, she holds key organizational roles, including Program and General Chair for the Optica Biophotonics Congress -OPTICA (2022-2025) and Associate Editor for Optics Express (2023-present). She also contributes as an expert reviewer for prestigious European grant programs (2024-present).
Kotsifaki holds a B.Sc. in Physics from the University of Patras, Greece, an M.Sc. in Physics of Laser Communications from the University of Essex, UK, and a Ph.D. in Physics from the National Technical University of Athens, Greece. Prior to her appointment at Duke Kunshan University, she was a Postdoctoral Scholar at the National Hellenic Research Foundation in Greece, a Postdoctoral Scholar at NTUA, and a Staff Scientist at the Okinawa Institute of Science and Technology Graduate University (OIST) in Japan.
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