A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited.

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2020-04-23

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Abstract

Premise:Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources-access to a microscope with 1000× magnification is the most limiting factor. Methods and Results:Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos. Conclusions:The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources.

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10.1002/aps3.11342

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Windham, MD, KM Pryer, DB Poindexter, F Li, CJ Rothfels and JB Beck (2020). A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited. Applications in plant sciences, 8(4). p. e11342. 10.1002/aps3.11342 Retrieved from https://hdl.handle.net/10161/21742.

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Pryer

Kathleen M. Pryer

Professor of Biology

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