A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited.
dc.contributor.author | Windham, MD | |
dc.contributor.author | Pryer, KM | |
dc.contributor.author | Poindexter, DB | |
dc.contributor.author | Li, F | |
dc.contributor.author | Rothfels, CJ | |
dc.contributor.author | Beck, JB | |
dc.date.accessioned | 2020-11-28T16:08:49Z | |
dc.date.available | 2020-11-28T16:08:49Z | |
dc.date.issued | 2020-04-23 | |
dc.date.updated | 2020-11-28T16:08:47Z | |
dc.description.abstract | Premise:Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources-access to a microscope with 1000× magnification is the most limiting factor. Methods and Results:Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos. Conclusions:The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources. | |
dc.identifier | APS311342 | |
dc.identifier.issn | 2168-0450 | |
dc.identifier.issn | 2168-0450 | |
dc.identifier.uri | ||
dc.language | eng | |
dc.publisher | John Wiley & Sons, Inc. | |
dc.relation.ispartof | Applications in plant sciences | |
dc.relation.isversionof | 10.1002/aps3.11342 | |
dc.subject | chromosome number | |
dc.subject | chromosome squash | |
dc.subject | cytology | |
dc.subject | meiosis | |
dc.subject | polyploidy | |
dc.subject | species complex | |
dc.title | A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited. | |
dc.type | Journal article | |
duke.contributor.orcid | Pryer, KM|0000-0002-9776-6736 | |
pubs.begin-page | e11342 | |
pubs.issue | 4 | |
pubs.organisational-group | Trinity College of Arts & Sciences | |
pubs.organisational-group | Biology | |
pubs.organisational-group | Duke Science & Society | |
pubs.organisational-group | Duke | |
pubs.organisational-group | Initiatives | |
pubs.organisational-group | Institutes and Provost's Academic Units | |
pubs.publication-status | Published | |
pubs.volume | 8 |
Files
Original bundle
- Name:
- A step-by-step protocol for meiotic chromosome counts in flowering plants A powerful and economical technique revisited.pdf
- Size:
- 475.54 KB
- Format:
- Adobe Portable Document Format