A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited.

dc.contributor.author

Windham, MD

dc.contributor.author

Pryer, KM

dc.contributor.author

Poindexter, DB

dc.contributor.author

Li, F

dc.contributor.author

Rothfels, CJ

dc.contributor.author

Beck, JB

dc.date.accessioned

2020-11-28T16:08:49Z

dc.date.available

2020-11-28T16:08:49Z

dc.date.issued

2020-04-23

dc.date.updated

2020-11-28T16:08:47Z

dc.description.abstract

Premise:Counting chromosomes is a fundamental botanical technique, yet it is often intimidating and increasingly sidestepped. Once mastered, the basic protocol can be applied to a broad range of taxa and research questions. It also reveals an aspect of the plant genome that is accessible with only the most basic of resources-access to a microscope with 1000× magnification is the most limiting factor. Methods and Results:Here we provide a detailed protocol for choosing, staining, and squashing angiosperm pollen mother cells. The protocol is supplemented by figures and two demonstration videos. Conclusions:The protocol we provide will hopefully demystify and reinvigorate a powerful and once commonplace botanical technique that is available to researchers regardless of their location and resources.

dc.identifier

APS311342

dc.identifier.issn

2168-0450

dc.identifier.issn

2168-0450

dc.identifier.uri

https://hdl.handle.net/10161/21742

dc.language

eng

dc.publisher

John Wiley & Sons, Inc.

dc.relation.ispartof

Applications in plant sciences

dc.relation.isversionof

10.1002/aps3.11342

dc.subject

chromosome number

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chromosome squash

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cytology

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meiosis

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polyploidy

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species complex

dc.title

A step-by-step protocol for meiotic chromosome counts in flowering plants: A powerful and economical technique revisited.

dc.type

Journal article

duke.contributor.orcid

Pryer, KM|0000-0002-9776-6736

pubs.begin-page

e11342

pubs.issue

4

pubs.organisational-group

Trinity College of Arts & Sciences

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Biology

pubs.organisational-group

Duke Science & Society

pubs.organisational-group

Duke

pubs.organisational-group

Initiatives

pubs.organisational-group

Institutes and Provost's Academic Units

pubs.publication-status

Published

pubs.volume

8

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