Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.

Feger, Bryan J

Thompson, J Will

Dubois, Laura G

Kommaddi, Reddy P

Foster, Matthew W

Mishra, Rajashree

Shenoy, Sudha K

Shibata, Yoichiro

Kidane, Yared H

Moseley, M Arthur

Carnell, Lisa S

Bowles, Dawn E

2018-12-28T17:18:38Z

2018-12-28T17:18:38Z

2016-09-27

2018-12-28T17:18:36Z

On Earth, biological systems have evolved in response to environmental stressors, interactions dictated by physical forces that include gravity. The absence of gravity is an extreme stressor and the impact of its absence on biological systems is ill-defined. Astronauts who have spent extended time under conditions of minimal gravity (microgravity) experience an array of biological alterations, including perturbations in cardiovascular function. We hypothesized that physiological perturbations in cardiac function in microgravity may be a consequence of alterations in molecular and organellar dynamics within the cellular milieu of cardiomyocytes. We used a combination of mass spectrometry-based approaches to compare the relative abundance and turnover rates of 848 and 196 proteins, respectively, in rat neonatal cardiomyocytes exposed to simulated microgravity or normal gravity. Gene functional enrichment analysis of these data suggested that the protein content and function of the mitochondria, ribosomes, and endoplasmic reticulum were differentially modulated in microgravity. We confirmed experimentally that in microgravity protein synthesis was decreased while apoptosis, cell viability, and protein degradation were largely unaffected. These data support our conclusion that in microgravity cardiomyocytes attempt to maintain mitochondrial homeostasis at the expense of protein synthesis. The overall response to this stress may culminate in cardiac muscle atrophy.

srep34091

2045-2322

2045-2322

https://hdl.handle.net/10161/17823

eng

Springer Science and Business Media LLC

Scientific reports

10.1038/srep34091

Science & Technology

Multidisciplinary Sciences

Science & Technology - Other Topics

SPECTROMETRY-BASED PROTEOMICS

UNFOLDED PROTEIN RESPONSE

ROTATING-WALL VESSEL

SIMULATED MICROGRAVITY

DYNAMIC SILAC

GENE

EXPRESSION

APOPTOSIS

TURNOVER

BIOLOGY

Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.

Journal article

Feger, Bryan J|0000-0002-5140-4414

Foster, Matthew W|0000-0003-0212-2346

Bowles, Dawn E|0000-0002-2781-1300

34091

1

School of Medicine

Duke

Medicine, Cardiology

Medicine

Clinical Science Departments

Duke Cancer Institute

Institutes and Centers

Cell Biology

Basic Science Departments

Surgery, Surgical Sciences

Surgery

Medicine, Pulmonary, Allergy, and Critical Care Medicine

Pharmacology & Cancer Biology

Published

6