Ex vivo expansion of murine and human hematopoietic stem cells.

dc.contributor.author

Doan, Phuong L

dc.contributor.author

Chute, John P

dc.coverage.spatial

United States

dc.date.accessioned

2017-04-28T15:08:33Z

dc.date.available

2017-04-28T15:08:33Z

dc.date.issued

2014

dc.description.abstract

Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety of the mature blood and immune system throughout the lifespan of an organism. Here, we describe methods to isolate and culture murine bone marrow (BM) CD34(-)ckit(+)Sca1(+)Lineage(-) (CD34(-)KSL) hematopoietic stem cells (HSCs). We also describe a method to measure functional HSC content via the competitive repopulation assay. Furthermore, we summarize methods to isolate and culture human CD34(+)CD38(-)Lineage(-) cells which are enriched for human hematopoietic stem and progenitor cells.

dc.identifier

https://www.ncbi.nlm.nih.gov/pubmed/25062631

dc.identifier.eissn

1940-6029

dc.identifier.uri

https://hdl.handle.net/10161/14159

dc.language

eng

dc.publisher

Springer New York

dc.relation.ispartof

Methods Mol Biol

dc.relation.isversionof

10.1007/978-1-4939-1133-2_14

dc.subject

Animals

dc.subject

Antigens, CD34

dc.subject

Antigens, CD38

dc.subject

Bone Marrow Cells

dc.subject

Cell Culture Techniques

dc.subject

Cell Lineage

dc.subject

Cell Separation

dc.subject

Fetal Blood

dc.subject

Hematopoietic Stem Cells

dc.subject

Humans

dc.subject

Mice

dc.title

Ex vivo expansion of murine and human hematopoietic stem cells.

dc.type

Journal article

duke.contributor.orcid

Doan, Phuong L|0000-0003-1361-2068

pubs.author-url

https://www.ncbi.nlm.nih.gov/pubmed/25062631

pubs.begin-page

211

pubs.end-page

221

pubs.organisational-group

Clinical Science Departments

pubs.organisational-group

Duke

pubs.organisational-group

Duke Cancer Institute

pubs.organisational-group

Institutes and Centers

pubs.organisational-group

Medicine

pubs.organisational-group

Medicine, Hematological Malignancies

pubs.organisational-group

School of Medicine

pubs.publication-status

Published

pubs.volume

1185

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
2014_Doan_ExVivoExpansion.pdf
Size:
363.56 KB
Format:
Adobe Portable Document Format
Description:
Published version