An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins
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<jats:p>Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously published MF isolation methods with the intent of determining which method was most suitable for MF protein isolation and solubilization. Here, we provide an optimized protocol to isolate sarcoplasmic and solubilized MF protein fractions from mammalian skeletal muscle suitable for several downstream assays.</jats:p>
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Roberts, Michael D, Kaelin C Young, Carlton D Fox, Christopher G Vann, Paul A Roberson, Shelby C Osburn, Johnathon H Moore, Petey W Mumford, et al. (n.d.). An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins. Journal of Biological Methods, 7(1). pp. e127–e127. 10.14440/jbm.2020.307 Retrieved from https://hdl.handle.net/10161/29766.
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Christopher Vann
Dr. Vann is an exercise physiologist with a research focus centered in skeletal muscle physiology. His research focuses on elucidating mechanisms of tissue-to-tissue crosstalk and understanding how exercise-induced changes in epigenetic, genetic, and protein-level factors relate to health and performance outcomes across the age span. As rates of obesity, cardiometabolic disease, and sarcopenia increase in the U.S., Dr. Vann's research is centered on understanding the role of exercise in improved health outcomes at the molecular level and applying this knowledge to develop precise evidence based exercise interventions.
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