(New) Methods for Detection of <i>Aspergillus fumigatus</i> Resistance in Clinical Samples.

dc.contributor.author

Jenks, Jeffrey D

dc.contributor.author

Spiess, Birgit

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Buchheidt, Dieter

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Hoenigl, Martin

dc.date.accessioned

2023-08-01T18:12:37Z

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2023-08-01T18:12:37Z

dc.date.issued

2019-09

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2023-08-01T18:12:36Z

dc.description.abstract

Purpose of review

The incidence of invasive aspergillosis has increased substantially over the past few decades, accompanied by a change in susceptibility patterns of Aspergillus fumigatus with increasing resistance observed against triazole antifungals, including voriconazole and isavuconazole, the most commonly used antifungal agents for the disease. Culture-based methods for determining triazole resistance are still the gold standard but are time consuming and lack sensitivity. We sought to provide an update on non-culture-based methods for detecting resistance patterns to Aspergillus.

Recent findings

New molecular-based approaches for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR) to detect mutations to the Cyp51A protein, have been developed which are able to detect most triazole-resistant A. fumigatus strains in patients with invasive aspergillosis.

Summary

Over the last few years, a number of non-culture-based methods for molecular detection of Aspergillus triazole resistance have been developed that may overcome some of the limitations of culture. These molecular methods are therefore of high epidemiological and clinical relevance, mainly in immunocompromised patients with hematological malignancies, where culture has particularly limited sensitivity. These assays are now able to detect most triazole-resistant Aspergillus fumigatus strains. Given that resistance rates vary, clinical utility for these assays still depends on regional resistance patterns.
dc.identifier.issn

1936-3761

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1936-377X

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https://hdl.handle.net/10161/28642

dc.language

eng

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Springer Science and Business Media LLC

dc.relation.ispartof

Current fungal infection reports

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10.1007/s12281-019-00342-w

dc.subject

Cyp51A gene

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Invasive aspergillosis

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Isavuconazole

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Triazoles

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Voriconazole

dc.title

(New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples.

dc.type

Journal article

duke.contributor.orcid

Jenks, Jeffrey D|0000-0001-6632-9587

pubs.begin-page

129

pubs.end-page

136

pubs.issue

3

pubs.organisational-group

Duke

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School of Medicine

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Clinical Science Departments

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Medicine

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Medicine, Infectious Diseases

pubs.publication-status

Published

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13

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